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电化学(中英文) ›› 2018, Vol. 24 ›› Issue (4): 303-308.  doi: 10.13208/j.electrochem.171229

• 研究论文 • 上一篇    下一篇

基于乙酰胆碱酯酶生物传感平台的西维因检测

张铭函1,2,姜俊巧3,葛君杰2,邢巍1,2*   

  1. 1. 暨南大学化学与材料学院,广东 广州 511400;2. 中国科学院长春应用化学研究所,吉林省先进化学电源实验室,吉林长春 130022;3. 长春工程学院理学院;吉林长春 130012
  • 收稿日期:2017-12-29 修回日期:2018-01-30 出版日期:2018-08-28 发布日期:2018-02-26
  • 通讯作者: 邢巍 E-mail:xingwei@ciac.ac.cn
  • 基金资助:
    获2017年中国科学院大学生科创计划资助

Acetylcholinesterase Biosensor Platform Based on BP2000 for the Detection of Carbaryl

ZHANG Ming-han1,2, JIANG Jun-qiao3, Ge Jun-jie2, XING Wei1,2*   

  1. 1.College of Chemistry and Material Science, Jinan University, Guangzhou 511400, China; 2.Jilin Laboratory of Advanced Power, Changchun Institute of Applied Chemistry,Changchun 130022, China; 3.School of Science, Changchun Institute of Technology, Changchun 130012, Jilin, China.
  • Received:2017-12-29 Revised:2018-01-30 Published:2018-08-28 Online:2018-02-26
  • Contact: XING Wei E-mail:xingwei@ciac.ac.cn

摘要: 为给农药西维因检测提供一种新方法,根据西维因抑制乙酰胆碱酯酶活性的原理,以黑珍珠2000(BP2000)为乙酰胆碱酯酶的固定化材料,采用滴凃电极法构建了基于乙酰胆碱酯酶的西维因生物传感平台. 结果表明,固定在BP2000 上的乙酰胆碱酯酶保持了对氯化乙酰胆碱的催化活性,并且由于BP2000 材料的引入,提升了电极有效的电化学活性表面积,而且电极上物质的电化学氧化拥有较低氧化电位(0.630 V)并伴随质子传输. 由BP2000 搭建成功的乙酰胆碱酯酶生物传感平台对西维因检测的线性响应范围为2.0 ng·mL-1 ~ 12.5 ng·mL-1,检测限为3.15 ng·mL-1. 本研究对酶生物传感平台和酶生物燃料电池体系中酶电极的构建提供了一种简单方法及高效载体.

关键词: 乙酰胆碱酯酶, 西维因, 酶生物传感器, 电化学, 黑珍珠2000

Abstract:

With the purpose of providing a new method for carbaryl (a pesticide) detection, on the basis of the principle that acetylcholinesterase (AChE) activity can be restrained by carbaryl, an AChE biosensor platform based on BP2000 (as a fixation) was constructed by dropping method. As a result, it revealed that AChE immobilized on BP2000 maintained its catalytic activity for acetylcholine (ATCl), and due to the introduction of the BP2000 material, the effective electrochemical surface area of the modified electrode was enlarged. In addition, the electrochemical oxidation at the modified electrode occurred at low potential (0.630 V) accompanied by proton transmission. The AChE biosensor platform based on BP2000 matrix for carbaryl detection was able to reflect a linear response in the range of 2.0 ng·mL-1 ~ 12.5 ng·mL-1 with the detection limit of 3.15 ng·mL-1. At last, this work will provide a simple method and an efficient matrix in establishing an enzyme electrode of enzymatic biosensor platform and enzymatic fuel cell.

Key words: acetylcholinesterase, carbaryl, enzyme-based biosensor, electrochemistry, black pearl 2000

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