酶法合成的葡萄糖氧化酶-纳米金复合物的直接电化学与生物传感

doi:10.13208/j.electrochem.140434

电化学（中英文） ›› 2014, Vol. 20 ›› Issue (6): 515-520. doi: 10.13208/j.electrochem.140434

• 生物电分析化学近期研究专辑（南京大学夏兴华教授主编） • 上一篇下一篇

酶法合成的葡萄糖氧化酶-纳米金复合物的直接电化学与生物传感

何芳¹，覃晓丽¹，傅迎春²，陈超¹，谢青季^1*，姚守拙¹

1. 湖南师范大学化学化工学院，化学生物学及中药分析教育部重点实验室，湖南长沙 410081； 2. 浙江大学生物系统工程与食品科学学院，浙江杭州 310029

收稿日期:2014-05-27 修回日期:2014-07-21 出版日期:2014-12-28 发布日期:2014-07-26
通讯作者: 谢青季 E-mail:xieqj@hunnu.edu.cn
基金资助:
国家自然科学基金项目（No. 21175042，No. 21075036）资助

Direct Electrochemistry and Glucose Biosensing of Glucose Oxidase-Gold Nanoparticles Composite Synthesized by Enzyme Method

HE Fang¹, QIN Xiao-li¹, FU Ying-chun², CHEN Chao¹, XIE Qing-ji^1*, YAO Shou-zhuo¹

1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China; 2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China

Received:2014-05-27 Revised:2014-07-21 Published:2014-12-28 Online:2014-07-26
Contact: XIE Qing-ji E-mail:xieqj@hunnu.edu.cn

摘要/Abstract

摘要： 将NaAuCl₄、葡萄糖氧化酶（GOx）和葡萄糖混合，借一步酶促反应制得吸附GOx的金纳米颗粒（AuNPs），再通过滴干修饰法研制了Nafion/GOx-AuNPs修饰的玻碳（GC）电极，并考察了该酶电极上GOx的直接电化学和生物传感性能. 这种酶法合成的GOx-AuNPs复合物有良好的酶直接电化学活性，也保持了GOx的生物活性，似可归因于酶法合成的纳米金更接近酶氧化还原活性中心的缘故. 该酶电极在-0.4 V（vs. SCE）电位下，其稳态电流下降与葡萄糖浓度（0.5 4 mmol·L^-1）成正比，检测下限0.2 mol·L^-1.

关键词: 葡萄糖氧化酶, 酶法合成, 葡萄糖氧化酶-纳米金复合物, 直接电化学, 生物传感

Abstract: Glucose oxidase (GOx)-gold nanoparticles (AuNPs) composite was synthesized by the one-pot enzyme reaction in the mixture solution of NaAuCl₄, GOx and glucose, and a Nafion/GOx-AuNPs/glassy carbon electrode was fabricated by the cast-coating method to examine the direct electrochemistry of GOx and the biosensing performance on this electrode. Such a GOx-AuNPs composite showed good direct-electrochemistry activity and bioactivity of GOx, probably because the enzyme-mediated AuNPs are close to the redox active centers of GOx. This enzyme electrode exhibited a linear amperometric response to glucose concentration (0.54 mmol·L^-1) at -0.4 V (vs. SCE), and a detection limit of 0.2 mol·L^-1 was obtained.

Key words: Glucose oxidase, enzymatic synthesis, glucose oxidase-Au nanoparticles composite, direct electrochemistry, biosensing

中图分类号:

O646

何芳，覃晓丽，傅迎春，陈超，谢青季*，姚守拙. 酶法合成的葡萄糖氧化酶-纳米金复合物的直接电化学与生物传感[J]. 电化学（中英文）, 2014, 20(6): 515-520.

HE Fang, QIN Xiao-li, FU Ying-chun, CHEN Chao, XIE Qing-ji*, YAO Shou-zhuo. Direct Electrochemistry and Glucose Biosensing of Glucose Oxidase-Gold Nanoparticles Composite Synthesized by Enzyme Method[J]. Journal of Electrochemistry, 2014, 20(6): 515-520.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.140434

https://electrochem.xmu.edu.cn/CN/Y2014/V20/I6/515

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酶法合成的葡萄糖氧化酶-纳米金复合物的直接电化学与生物传感

Direct Electrochemistry and Glucose Biosensing of Glucose Oxidase-Gold Nanoparticles Composite Synthesized by Enzyme Method

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