二茂铁功能化Fe3O4/碳纳米管/壳聚糖复合膜葡萄糖生物传感电极的研究

doi:10.13208/j.electrochem.121108

电化学（中英文） ›› 2014, Vol. 20 ›› Issue (1): 33-38. doi: 10.13208/j.electrochem.121108

二茂铁功能化Fe₃O₄/碳纳米管/壳聚糖复合膜葡萄糖生物传感电极的研究

彭花萍¹，查代君²，黄郑隽¹，陈伟¹，刘爱林^1*，林新华^1*

1. 福建医科大学药学院药物分析系，福建福州 350004；2. 南昌大学高等研究院，江西南昌 330031

收稿日期:2012-11-08 修回日期:2013-01-25 出版日期:2014-02-25 发布日期:2014-02-24
通讯作者: 刘爱林，林新华 E-mail:ailinliu@mail.fjmu.edu.cn; xhl1963@sina.com
基金资助:
国家863计划资助项目(No. 2012AA022604)、国家自然科学基金（No. 20975021，No. 21275028）、福建省高校产学研科技重点项目(No. 2010Y4003)、福建省自然科学基金资助项目(No. 2010J06011)和福建医科大学博士启动基金（No. 2011BS005）资助

Design and Development of a Novel Glucose Biosensor Based on the Ferrocene-Functionalized Fe₃O₄ Nanoparticles/Carbon Nanotubes/Chitosan Nanocomposite Film Modified Electrode

PENG Hua-ping¹，ZHA Dai-Jun²，CHEN Wei¹，LIU Ai-lin^1*，LIN Xin-hua^1*

1. Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；2. Institute for Advanced Study of Nanchang University，Nanchang 330031，China

Received:2012-11-08 Revised:2013-01-25 Published:2014-02-25 Online:2014-02-24
Contact: LIU Ai-lin，LIN Xin-hua E-mail:ailinliu@mail.fjmu.edu.cn; xhl1963@sina.com

摘要/Abstract

摘要： 采用交联法制备了羧基二茂铁功能化Fe₃O₄纳米粒子（FMC-AFNPs）复合材料，并将该复合纳米材料与多壁碳纳米管（MWNTs）、壳聚糖（CS）及葡萄糖氧化酶（GOD）混合修饰于自制的磁性玻碳基底(MGC)表面，制备了GOD/FMC-AFNPs/MWNTs/CS复合膜生物传感器电极. 实验结果表明，FMC-AFNPs复合材料有效地克服了二茂铁在电极表面的泄漏，且FMC-AFNPs/MWNTs/CS复合膜良好的生物兼容性较大地改善了固定化GOD的生物活性. MWNTs具有良好的导电性和大比表面积，在修饰膜内可作为电子传递“导线”，极大地促进电极的电子传递速率，提高电极的电催化活性和灵敏度. 该电极的葡萄糖检测的线性范围为1.0×10^-5 ~ 6.0×10^-3 molL^-1，检测限为3.2×10^-6 mmolL^-1（S/N=3），表观米氏常数为5.03×10^-3 mmolL^-1，且有较好的稳定性和重现性.

关键词: 二茂铁修饰Fe₃O₄, 碳纳米管, 壳聚糖, 葡萄糖氧化酶, 生物传感器

Abstract: A novel platform for the fabrication of glucose biosensor was successfully constructed by entrapping glucose oxidase (GOD) in a ferrocene monocarboxylic acid-aminated Fe₃O₄ magnetic nanoparticles conjugate (FMC-AFNPs)/chitosan (CS)/multiwall carbon nanotubes (MWNTs) nanocomposite. The formation of FMC-AFNPs could effectively prevent the leakage of ferrocene and retain its electrochemical activity. This GOD/FMC-AFNPs/CS/MWNTs matrix provided a biocompatible microenvironment for retaining the native activity of the immobilized GOD. Moreover, the presence of MWNTs enhanced the charge-transport properties of the composite and facilitated electron transfer between the GOD and the electrode for the electrocatalysis of glucose. Under the optimal conditions the designed biosensor to glucose exhibited a wide and useful linear range of 1.0×10^-5 to 6.0×10^-3 molL^-1 with a low detection limit of 3.2×10^-6 molL^-1（S/N=3）. The value of was 5.03×10^-3 molL^-1, indicating that the biosensor possesses higher biological affinity to glucose. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.

Key words: ferrocene-modified Fe₃O₄ nanoparticles, carbon nanotubes, chitosan, glucose oxidase, biosensor

中图分类号:

O657.1

彭花萍，查代君，黄郑隽，陈伟，刘爱林*，林新华*. 二茂铁功能化Fe₃O₄/碳纳米管/壳聚糖复合膜葡萄糖生物传感电极的研究[J]. 电化学（中英文）, 2014, 20(1): 33-38.

PENG Hua-ping，ZHA Dai-Jun，CHEN Wei，LIU Ai-lin*，LIN Xin-hua*. Design and Development of a Novel Glucose Biosensor Based on the Ferrocene-Functionalized Fe₃O₄ Nanoparticles/Carbon Nanotubes/Chitosan Nanocomposite Film Modified Electrode[J]. Journal of Electrochemistry, 2014, 20(1): 33-38.

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二茂铁功能化Fe₃O₄/碳纳米管/壳聚糖复合膜葡萄糖生物传感电极的研究

Design and Development of a Novel Glucose Biosensor Based on the Ferrocene-Functionalized Fe₃O₄ Nanoparticles/Carbon Nanotubes/Chitosan Nanocomposite Film Modified Electrode

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