1-芘丁酸/石墨烯复合物的电化学性质及其在葡萄糖传感器上的应用

doi:10.61558/2993-074X.2615

电化学（中英文） ›› 2012, Vol. 18 ›› Issue (5): 450-456. doi: 10.61558/2993-074X.2615

1-芘丁酸/石墨烯复合物的电化学性质及其在葡萄糖传感器上的应用

王敏，王炯，王凤彬，夏兴华^*

南京大学化学化工学院，生命分析化学国家重点实验室，江苏南京 210093

收稿日期:2012-06-26 修回日期:2012-08-16 出版日期:2012-10-28 发布日期:2012-10-28
通讯作者: 夏兴华 E-mail:xhxia@nju.edu.cn
基金资助:
This work was financially supported by the National 973 Basic Research Program (No. 2012CB933804), the National Natural Science Foundation of China(No. 21035002, No. 21121091) and the Natural Science Foundation of Jiangsu province (BK2010009)

The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors

Min Wang, Jiong Wang, Fengbin Wang, Xinghua Xia^*

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

Received:2012-06-26 Revised:2012-08-16 Published:2012-10-28 Online:2012-10-28
Contact: Xinghua Xia E-mail:xhxia@nju.edu.cn
Supported by:
This work was financially supported by the National 973 Basic Research Program (No. 2012CB933804), the National Natural Science Foundation of China(No. 21035002, No. 21121091) and the Natural Science Foundation of Jiangsu province (BK2010009)

摘要/Abstract

摘要： 本文采用一步法制备了1-芘丁酸/石墨烯复合物(PBA/G)，研究了其电化学性质. 采用铁氰化钾和亚铁氰化钾电化学探针测定了电化学阻抗滴定曲线，确定了PBA/G的表观pK_a为6.2. 此外，将葡萄糖氧化酶(GOD)共价键合在PBA/G表面构建了葡萄糖电化学传感器，其电化学响应与葡萄糖浓度（5 mmol L^-1浓度范围内）呈线性，检测限为0.085 mmol L^-1. 实验还测定了固定在PBA/G表面的GOD的表观米氏常数为5.40 mmol L^-1，表明固定化的GOD对葡萄糖有较高的催化活性。

关键词: 1-芘丁酸, 石墨烯, 葡萄糖氧化酶, 电化学生物传感器, 葡萄糖

Abstract: The electrochemical properties of 1-pyrenebutyric acid/graphene composites (PBA/G) obtained by one-step synthesis via π-π stacking was investigated. The electrochemical impedance titration curve shows the surface charge changes as function of solution pH by using ferricyanide/ferrocyanide redox couple as the probe. An apparent pK_a value is estimated as 6.2 according to the impedance titration curve. In addition, a glucose biosensor was constructed by immobilizing glucose oxidase (GOD) on the surface of PBA/G via covalent interaction. This biosensor shows a linear response to glucose within the concentration up to 5 mmol L^-1 with a detection limit of 0.085 mmol L^-1. A small apparent Michaelis-Menten constant (5.40 mmol L^-1) of the immobilized GOD suggests that the immobilized GOD retains its bioactivity and shows high catalytic activity to glucose.

Key words: 1-pyrenebutyric acid, graphene, glucose oxidase, electrochemical biosensor, glucose

中图分类号:

O646

王敏, 王炯, 王凤彬, 夏兴华. 1-芘丁酸/石墨烯复合物的电化学性质及其在葡萄糖传感器上的应用[J]. 电化学（中英文）, 2012, 18(5): 450-456.

Min Wang, Jiong Wang, Fengbin Wang, Xinghua Xia. The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors[J]. Journal of Electrochemistry, 2012, 18(5): 450-456.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.61558/2993-074X.2615

https://electrochem.xmu.edu.cn/CN/Y2012/V18/I5/450

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1-芘丁酸/石墨烯复合物的电化学性质及其在葡萄糖传感器上的应用

The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors

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