关键词: 葡萄糖氧化酶, 铂纳米线, 硫堇, 生物传感器, 电催化

Abstract: A new amperometric biosensor based on Nafion, thionine and latinum nanowires was developed for sensitive detection of glucose. Initially, positively charged thionine molecules were immobilized onto the surface of Nafion-modified glassy carbon electrode through the interaction between -SO₃^- and -NH₃⁺, and glucose oxidase was then assembled on the modified electrode by using platinum nanowires as the cross-linkage. The preparation process was characterized by using cyclic voltammetry. Two enzymatic biosensors modified with and without platinum nanowires were used for the detection of glucose, and the improved analytical properties were obtained with platinum nanowires. Experimental results indicated that the as-prepared biosensor exhibited good electrocatalytic response toward glucose in the dynamic range of 1.0 × 10^-5 ~ 6.0 × 10^-3 mol·L^-1 with a detection limit of 3.0 × 10^-6 mol·L^-1 at S/N =3. Moreover, the reproducibility and stability of the biosensor were acceptable.

Key words: glucose oxidase, platinum nanowires, thionine, biosensors, electrochemical catalysis