氯过氧化物酶-聚L-赖氨酸/GC电极的电化学特性

doi:10.61558/2993-074X.2040

摘要/Abstract

摘要： 应用电化学方法在玻碳电极上修饰聚L-赖氨酸膜,以1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐做交联剂,固定氯过氧化物酶.修饰电极的循环伏安曲线呈现一对可逆的氧化还原峰,表明聚L-赖氨酸能够很好地促进氯过氧化物酶在电极表面的直接电子传递,这是一个受吸附控制并伴随有质子转移的准可逆电子传递过程.该电极有很好的稳定性,并能显著地电催化氧的电化学还原反应.

关键词: 氯过氧化物酶, L-赖氨酸, 电聚合, 直接电化学, 电催化, 修饰电极

Abstract: Poly-L-lysine(PLL) was electropolymerized onto the surface of glassy carbon(GC) electrode.1[(3-dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride(EDC) was used as a Cross-linking agent to immobilized Chloroperoxidase(CPO) onto the PLL/GC electrode.A pair of well-defined redox peaks was observed at CPO-PLL/GC electrode,which clearly suggested that PLL can promote the direct electron transfer between the CPO and the GC electrodes.The cyclic voltammetry results also showed that the electron transfer at CPO-PLL/GC is a surface adsorption-controlled quasi-reversible process,which is accompanied with proton transfer.The CPO-PLL/GC electrode exhibited a good electrochemical stability and excellently electrocatalytic activity for electrochemical reduction of O2.

Key words: chloroperoxidase, poly-L-lysine, electropolymerization, direct electrochemistry, electrocatalysis, modified electrode

中图分类号:

O646.54

赵雯, 吴霞琴, 陆中庆, 后雯璟, 李和兴, . 氯过氧化物酶-聚L-赖氨酸/GC电极的电化学特性[J]. 电化学（中英文）, 2010, 16(1): 65-69.

ZHAO Wen, WU Xia-qin, LU Zhong-qing, HOU Wen-jing, LI He-xing. The Electrochemical Characteristics of CPO-PLL/GC Electrode[J]. Journal of Electrochemistry, 2010, 16(1): 65-69.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.61558/2993-074X.2040

https://electrochem.xmu.edu.cn/CN/Y2010/V16/I1/65

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