共反应试剂增强电致化学发光信号生物传感器

doi:10.13208/j.electrochem.140439

摘要/Abstract

摘要： 在电致化学发光（ECL）生物传感器的构建中，利用共反应试剂促进发光基团的发光效率是一种常见、方便且非常有效的方法. 然而，如何更好地利用共反应试剂使其更加有效地与发光基团作用是提高该类生物传感器灵敏度的重要因素. 本文结合作者课题组部分工作综述了三种共反应试剂放大ECL信号的构建：共反应试剂内置于检测底液；共反应试剂共存于电极表面；酶促生成共反应试剂，并提出了今后ECL信号放大构建的展望.

关键词: 电致化学发光, 共反应试剂, 信号放大, 生物传感器

Abstract: Using co-reactants to enhance the luminous efficiency of luminophores is a common, convenient and effective method in the construction of the electrochemiluminescence (ECL) biosensors. However, the different methods of introducing co-reactants in the construction of the ECL biosensors will bring different amplification effects. In this review, several signal amplification methods through co-reactants are summarized: 1) Adding the co-reactants directly into the detection solution; 2) Immobilizing the co-reactants onto the electrode surface; 3) Using enzymatic reaction to generate co-reactants in situ around the electrode surface. And then, the outlook of the ECL signal amplification strategy is proposed on those bases.

Key words: electrochemiluminescence, co-reactants, amplification, biosensors

中图分类号:

O646

王海军，肖丽娟，何颖，蒋欣亚，袁亚利，卓颖，柴雅琴，袁若*. 共反应试剂增强电致化学发光信号生物传感器[J]. 电化学（中英文）, 2015, 21(1): 13-21.

WANG Hai-jun, XIAO Li-juan, HE Ying, JIANG Xin-ya, YUAN Ya-li, ZHUO Ying, CHAI Ya-qin, YUAN Ruo*. Electrochemiluminescence Biosensors Based on the Signal Amplification of Co-reactants[J]. Journal of Electrochemistry, 2015, 21(1): 13-21.

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