Mb/AuNPs/MWNTs/GC电极对H2O2电催化性能的研究

doi:10.61558/2993-074X.2934

电化学（中英文） ›› 2012, Vol. 18 ›› Issue (4): 377-382. doi: 10.61558/2993-074X.2934

Mb/AuNPs/MWNTs/GC电极对H₂O₂电催化性能的研究

黄郑隽¹, 彭花萍^1*, 查代君², 刘爱林¹, 陈伟¹, 林新华¹, 游勇基^1,3*

1. 福建医科大学药学院药物分析系，福建福州 350004； 2. 南昌大学高等研究院，江西南昌 330031；3. 福建省药品检验所，福建福州350001

收稿日期:2012-02-25 修回日期:2012-04-10 出版日期:2012-08-28 发布日期:2012-04-15
通讯作者: 彭花萍,游勇基 E-mail:penghuaping2006@126.com；youyj@sina.com
基金资助:
国家863计划资助项目(No. 2008AA02Z433)，国家自然科学基金资助项目(No. 20975021，No. 81171668)，福建省高校产学研科技重点项目(No. 2010Y4003)，福建省自然科学基金资助项目(No. 2010J06011)，福建医科大学校重大项目(No. 09ZD013)，福建省教育厅科技项目(No. JA10126，No. JA11110)和福建医科大学博士启动基金（No. 2011BS005）资助

Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction

HUANG Zheng-Juan¹, PENG Hua-Ping^1*, CHA Dai-Jun,² LIU Ai-Lin¹, CHEN Wei¹, LIN Xin-Hua¹, YOU Yong-Ji^1,3*

1. Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；2. Institute for Advanced Study of Nanchang University，Nanchang 330031，China；3. Fujian Provincial Institute for Drug Control，Fuzhou 350001，China

Received:2012-02-25 Revised:2012-04-10 Published:2012-08-28 Online:2012-04-15
Contact: PENG Hua-Ping, YOU Yong-Ji E-mail:penghuaping2006@126.com；youyj@sina.com

摘要/Abstract

摘要： 采用还原法制备了AuNPs/MWNTs复合材料，并构建了氧化还原蛋白质的固定化和生物传感界面AuNPs/MWNTs/GC电极. 以肌红蛋白（Myoglobin，Mb）为例，研究了固定化蛋白质在AuNPs/MWCNTs/GC电极上的直接电化学. 结果表明，AuNPs/MWCNTs复合材料不仅能有效地促进Mb与电极表面的直接电子转移，而且能很好地保持固定化Mb的生物催化活性. Mb/AuNPs/MWCNTs/GC电极对H2O2具有良好的电催化还原性能，其线性响应范围为1 ~ 138 μmol.L-1，检测下限为0.32 μmol.L-1（S/N=3），并具有较低的米氏常数（0.143 mmol.L-1）. 该电极操作简单，响应迅速，稳定性和重现性好，有望用于蛋白质的固定化及第三代生物传感器的制备.

关键词: 肌红蛋白, AuNPs/MWCNTs, 直接电化学, 生物传感器

Abstract: A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial (AuNPs/MWNTs), for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin (Mb) as a model, the direct electrochemistry of the immobilized proteins on the AuNPs/MWNTs composite was studied. The results showed that the AuNPs/MWNTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/MWNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H₂O₂ with a linear range of 1 ~ 138 μmol.L^-1，low detection limit of 0.32 μmol.L^-1 (S/N=3) and a low apparent K_m^app value of 0.143 mmol.L^-1. The simple operation, fast response and well reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.

Key words: myoglobin, AuNPs/MWNTs composite, direct electrochemistry, biosensor

中图分类号:

O657.1

黄郑隽, 彭花萍, 查代君, 刘爱林, 陈伟, 林新华, 游勇基. Mb/AuNPs/MWNTs/GC电极对H₂O₂电催化性能的研究[J]. 电化学（中英文）, 2012, 18(4): 377-382.

HUANG Zheng-Juan, PENG Hua-Ping, CHA Dai-Jun, LIU Ai-Lin, CHEN Wei, LIN Xin-Hua, YOU Yong-Ji. Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction[J]. Journal of Electrochemistry, 2012, 18(4): 377-382.

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Mb/AuNPs/MWNTs/GC电极对H₂O₂电催化性能的研究

Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction

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