基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究

doi:10.61558/2993-074X.2086

电化学（中英文） ›› 2011, Vol. 17 ›› Issue (2): 180-185. doi: 10.61558/2993-074X.2086

基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究

汪红梅，林丽清，翁少煌，王丽满，刘银环，林新华*

福建医科大学药学院，福建福州 350004

收稿日期:2010-12-07 修回日期:2011-01-28 出版日期:2011-05-28 发布日期:2011-05-06
通讯作者: 林新华 E-mail:xinhua63@163.com
基金资助:
国家自然科学基金项目(20675015)，国家863计划(No. 2008AA02Z433)，福建省自然科学基金(No.2010J06011, 2010J01032)，福建省教育厅（No.JA10155），福建省高校产学研科技重点项目(2010Y4003)

Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene

WANG Hong-mei, LIN Li-qing,WENG Shao-huang,WANG Li-man, LIU Yin-huan, LIN Xin-hua*

College of Pharmacy, Fujian Medical University, Fuzhou 350004,China

Received:2010-12-07 Revised:2011-01-28 Published:2011-05-28 Online:2011-05-06
Contact: LIN Xin-hua E-mail:xinhua63@163.com

摘要/Abstract

摘要： 基于慢性粒细胞白血病中BCR/ABL融合基因的碱基序列，设计了一种新型发夹结构锁核酸(locked nucleic acids, LNA)探针，把LNA探针通过Au-s键固定在金电极表面构建了特异的生物传感器.LNA探针与目标链DNA杂交，以自行合成的苯甲酸二聚铜配合物([Cu2(C7H5O2)4(C2H6O)2], 简称[Cu(R)2]2+)为杂交指示剂，应用差示脉冲伏安法进行检测，表现出良好的响应信号.该新型锁核酸传感器能较好的区分完全互补链DNA、单碱基错配链DNA.对互补链DNA检测的线性范围为1.0×10-8～1.0×10-6 mol•L-1，检出限为2.0×10-9 mol•L-1.

关键词: 发夹锁核酸探针, 生物传感器, BCR/ABL融合基因, 苯甲酸二聚铜配合物

Abstract: In this article, a new kind of structure hairpin LNA probe was designed to detect BCR/ABL fusion gene in Chronic Myelocytic Leukemia. The LNA probe was immobilized on the gold electrode(AuE) through sulfur–Au interaction to construct specific electrochemical biosensor. The electrochemical response of the sensor to hybridization of the LNA probe with the target DNA was studied using [Cu(R)2]2+ as an electrochemical indicator. The optimal condition was discussed. The experimental results indicated that in pH 7.4 PBS buffer solution, this new method has excellent specificity for single base mismatch and complementary after hybridization. The relationship between the increased oxidation peak current of [Cu(R)2]2+ and the concentration of complementary strand was linear in the range of 1.0×10-8~1.0×10-6 mol/L. The detection limit was 2.0×10-9 mol/L.

Key words: hairpin LNA probe, electrochemical biosensor, BCR/ABL fusion gene, [Cu2(C7H5O2)4(C2H6O)2]

中图分类号:

O646

汪红梅, 林丽清, 翁少煌, 王丽满, 刘银环, 林新华. 基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究[J]. 电化学（中英文）, 2011, 17(2): 180-185.

WANG Hong-mei, LIN Li-qing, WENG Shao-huang, WANG Li-man, LIU Yin-huan, LIN Xin-hua. Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene[J]. Journal of Electrochemistry, 2011, 17(2): 180-185.

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https://electrochem.xmu.edu.cn/CN/Y2011/V17/I2/180

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基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究

Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene

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