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电化学(中英文) ›› 2014, Vol. 20 ›› Issue (1): 33-38.  doi: 10.13208/j.electrochem.121108

• 研究论文 • 上一篇    下一篇

二茂铁功能化Fe3O4/碳纳米管/壳聚糖复合膜葡萄糖生物传感电极的研究

彭花萍1,查代君2,黄郑隽1,陈伟1,刘爱林1*,林新华1*   

  1. 1. 福建医科大学药学院药物分析系,福建 福州 350004;2. 南昌大学高等研究院,江西 南昌 330031
  • 收稿日期:2012-11-08 修回日期:2013-01-25 出版日期:2014-02-25 发布日期:2014-02-24
  • 通讯作者: 刘爱林,林新华 E-mail:ailinliu@mail.fjmu.edu.cn; xhl1963@sina.com
  • 基金资助:

    国家863计划资助项目(No. 2012AA022604)、国家自然科学基金(No. 20975021,No. 21275028)、福建省高校产学研科技重点项目(No. 2010Y4003)、福建省自然科学基金资助项目(No. 2010J06011)和福建医科大学博士启动基金(No. 2011BS005)资助

Design and Development of a Novel Glucose Biosensor Based on the Ferrocene-Functionalized Fe3O4 Nanoparticles/Carbon Nanotubes/Chitosan Nanocomposite Film Modified Electrode

PENG Hua-ping1,ZHA Dai-Jun2,CHEN Wei1,LIU Ai-lin1*,LIN Xin-hua1*   

  1. 1. Department of Pharmaceutical Analysis of the Fujian Medical University,Fuzhou 350004,China;2. Institute for Advanced Study of Nanchang University,Nanchang 330031,China
  • Received:2012-11-08 Revised:2013-01-25 Published:2014-02-25 Online:2014-02-24
  • Contact: LIU Ai-lin,LIN Xin-hua E-mail:ailinliu@mail.fjmu.edu.cn; xhl1963@sina.com

摘要: 采用交联法制备了羧基二茂铁功能化Fe3O4纳米粒子(FMC-AFNPs)复合材料,并将该复合纳米材料与多壁碳纳米管(MWNTs)、壳聚糖(CS)及葡萄糖氧化酶(GOD)混合修饰于自制的磁性玻碳基底(MGC)表面,制备了GOD/FMC-AFNPs/MWNTs/CS复合膜生物传感器电极. 实验结果表明,FMC-AFNPs复合材料有效地克服了二茂铁在电极表面的泄漏,且FMC-AFNPs/MWNTs/CS复合膜良好的生物兼容性较大地改善了固定化GOD的生物活性. MWNTs具有良好的导电性和大比表面积,在修饰膜内可作为电子传递“导线”,极大地促进电极的电子传递速率,提高电极的电催化活性和灵敏度. 该电极的葡萄糖检测的线性范围为1.0×10-5 ~ 6.0×10-3 molL-1,检测限为3.2×10-6 mmolL-1(S/N=3),表观米氏常数为5.03×10-3 mmolL-1,且有较好的稳定性和重现性.

关键词: 二茂铁修饰Fe3O4, 碳纳米管, 壳聚糖, 葡萄糖氧化酶, 生物传感器

Abstract: A novel platform for the fabrication of glucose biosensor was successfully constructed by entrapping glucose oxidase (GOD) in a ferrocene monocarboxylic acid-aminated Fe3O4 magnetic nanoparticles conjugate (FMC-AFNPs)/chitosan (CS)/multiwall carbon nanotubes (MWNTs) nanocomposite. The formation of FMC-AFNPs could effectively prevent the leakage of ferrocene and retain its electrochemical activity. This GOD/FMC-AFNPs/CS/MWNTs matrix provided a biocompatible microenvironment for retaining the native activity of the immobilized GOD. Moreover, the presence of MWNTs enhanced the charge-transport properties of the composite and facilitated electron transfer between the GOD and the electrode for the electrocatalysis of glucose. Under the optimal conditions the designed biosensor to glucose exhibited a wide and useful linear range of 1.0×10-5 to 6.0×10-3 molL-1 with a low detection limit of 3.2×10-6 molL-1(S/N=3). The value of was 5.03×10-3 molL-1, indicating that the biosensor possesses higher biological affinity to glucose. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.

Key words: ferrocene-modified Fe3O4 nanoparticles, carbon nanotubes, chitosan, glucose oxidase, biosensor

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