基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究

doi:10.13208/j.electrochem.160829

电化学（中英文） ›› 2017, Vol. 23 ›› Issue (1): 53-58. doi: 10.13208/j.electrochem.160829

基于WS₂量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究

李晨露¹，彭花萍²*，黄种南²，盛依伦²，吴佩文³*，林新华²

1. 福建医科大学附属第一医院高压氧室，福建福州 350005; 2. 福建医科大学药学院药物分析系，福建福州 350122; 3. 福建医科大学附属第一医院内分泌科，福建福州 350005

收稿日期:2016-08-29 修回日期:2016-09-23 出版日期:2017-02-28 发布日期:2016-10-21
通讯作者: 彭花萍,吴佩文 E-mail:phpfjmu@126.com, 247056892@qq.com
基金资助:
国家自然科学基金项目（No.21405015, No.81400805），福建省自然科学基金资助项目（No.2014J05092, No.2016J01449），大学生创新创业训练项目基金（No.C1634）资助

Direct Electrochemistry of Glucose Oxidase Based on WS₂ Quantum Dots and its Biosensing Application

LI Chen-lu¹, PENG Hua-ping²*, HUANG Zhong-nan², SHENG Yi-lun², WU Pei-wen³*, LIN Xin-hua²

1. Department of high pressure oxygen,, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005 , China;2. Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350122，China；3. Department of Endocrinology, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005 , China

Received:2016-08-29 Revised:2016-09-23 Published:2017-02-28 Online:2016-10-21
Contact: PENG Hua-ping,WU Pei-wen E-mail:phpfjmu@126.com, 247056892@qq.com

摘要/Abstract

摘要：

采用水热法制备水溶性WS₂量子点（WS₂QDs）材料，并将该材料进一步用于葡萄糖氧化酶（GOx）的有效固定，构建GOx/W₂QDs/GCE传感界面. 采用透射电镜、紫外-可见光谱和电化学等方法对材料的形貌、GOx的固定化过程，以及传感器的直接电化学和电催化性能进行了表征. 结果表明，WS₂QDs材料能够有效促进GOx与电极之间的直接电子转移. 并且，基于该传感器对葡萄糖良好的电催化作用，该方法有效实现了对葡萄糖的高灵敏检测，其线性范围为25 ~ 100 μmol·L^-1和100 ~ 600 μmol·L^-1，检测限为5.0 μmol·L^-1（S/N=3）. 该传感器具有良好的选择性、重现性和稳定性，可用于实际样品血糖的分析测定.

关键词: WS₂ 量子点, 葡萄糖氧化酶, 直接电化学, 生物传感器

Abstract:

In this study, a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase (GOx) on tungsten disulfide quantum dots (WS₂QDs) on the surface of glassy carbon electrode (GCE). Transmission electron microscopy, UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology, structure, and electrochemical behaviors of the as-prepared WS₂ QDs and the biofilm modified electrode. The results suggested that the WS₂QDs could accelerate the electron transfer between the electrode and the immobilized enzyme, which enabled the direct electrochemistry of GOx without any electron mediator. Besides, the immobilized GOx in WS₂QDsfilm exhibited excellent electrocatalytic activity toward oxidation of glucose due to the excellent biocompatibility of the WS₂QDs. The proposed GOx/WS₂QDs biofilm electrode exhibited a linear response to glucose concentration in the ranges of 25 ~ 100 μmol·L^-1 and 100 ~ 600 μmol·L^-1, and the detection limit of the biosensor was as low as 5.0 μmol·L^-1. The biosensor also exhibited good selectivity, reproducibility and long-term stability, which might be potenially used for the detection of the real samples.

Key words: tungsten disulfide quantum dots, glucose oxidase, direct electrochemistry, biosensor

中图分类号:

O657.1

李晨露，彭花萍，黄种南，盛依伦，吴佩文，林新华. 基于WS₂量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究[J]. 电化学（中英文）, 2017, 23(1): 53-58.

LI Chen-lu, PENG Hua-ping, HUANG Zhong-nan, SHENG Yi-lun, WU Pei-wen, LIN Xin-hua. Direct Electrochemistry of Glucose Oxidase Based on WS₂ Quantum Dots and its Biosensing Application[J]. Journal of Electrochemistry, 2017, 23(1): 53-58.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.160829

https://electrochem.xmu.edu.cn/CN/Y2017/V23/I1/53

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基于WS₂量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究

Direct Electrochemistry of Glucose Oxidase Based on WS₂ Quantum Dots and its Biosensing Application

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