孔蛋白-磷脂仿生膜的葡萄糖氧化酶电化学

doi:10.13208/j.electrochem.131176

电化学（中英文） ›› 2014, Vol. 20 ›› Issue (5): 439-443. doi: 10.13208/j.electrochem.131176

• 电化学近期研究专辑（厦门大学姜艳霞教授主编） • 上一篇下一篇

孔蛋白-磷脂仿生膜的葡萄糖氧化酶电化学

王琨琦^1,2，张作明^3*，邢巍^1*，刘长鹏¹，周星²

1. 中国科学院长春应用化学研究所，吉林长春 130022；2. 长春工程学院，吉林长春 130012；3. 吉林大学，吉林长春 130012

收稿日期:2014-01-22 修回日期:2014-04-09 出版日期:2014-10-28 发布日期:2014-04-14
通讯作者: 张作明，邢巍 E-mail:xingwei@ciac.ac.cn; zmzhang@jlu.edu.cn
基金资助:
国家973计划项目（No. Y11J032001）资助

Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane

WANG Kun-qi^1,2, ZHANG Zuo-ming^3*, XING Wei^1*, LIU Chang-peng1, ZHOU Xing²

1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; 2. Changchun Institute of Technology, Changchun 1300122, China; 3. Jilin University, Changchun 130012, China

Received:2014-01-22 Revised:2014-04-09 Published:2014-10-28 Online:2014-04-14
Contact: ZHANG Zuo-ming, XING Wei E-mail:xingwei@ciac.ac.cn; zmzhang@jlu.edu.cn

摘要/Abstract

摘要： 采用孔蛋白（MspA）和双肉豆蔻磷脂酰胆碱（DMPC）在玻碳（GC）基底表面成功构建有仿生特性的纳米通道膜，同时将葡萄糖氧化酶（GOD）修饰于膜上. 使用循环伏安法研究GOD/MspA-DMPC/GC电极的GOD直接电化学过程以及其对氧气和葡萄糖的响应. 研究发现，MspA与DMPC形成的仿生纳米通道膜内，GOD在接近生物体系FAD/FADH标准电位处实现了自身两质子、两电子表面控制的电化学反应. MspA与DMPC的仿生纳米通道膜体系为GOD提供了理想活性环境.

关键词: 孔蛋白, 仿生膜, 葡萄糖氧化酶, 电化学, 双肉豆蔻磷脂酰胆碱

Abstract: Biomimic membrane with nano-channels, which is made up of porin MspA and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is constructed on glassy carbon substrate, and glucose oxidase (GOD) is modified on it. The direct electrochemical reaction and electrocatalytic behavior to oxygen and glucose of GOD on the GOD/MspA-DMPC/GC electreode are expounded by the cyclic voltammetric method. The study shows that GOD immobilized on porin MspA and DMPC biomimic membrane displays direct and surface-controlled electrochemical reaction nearby formal potential (E0′) of the flavoprotein active centre (FAD/FADH), and the electrochemical reaction contains two electrons and two protons exchange in 0.1 mmol?L^-1 phosphate buffer solution (PBS) (pH 7.0). Furthermore, it is also discovered that, GOD immobilized on porin MspA and DMPC biomimic membrane possesses an excellent bioelectrocatalytic activity for the reduction of O₂ and the oxidation of glucose. That is to say, the biomimic nano-channels membrane formed by porin MspA and DMPC provides an ideal living environment for GOD. So, the GOD/MspA-DMPC/GC electrode can be utilized in biosensor and biofuel cell in the future.

Key words: porin MspA, biomimic membrane, glucose oxidase, electrochemistry, 1,2-dimyristoyl-sn-glycero-3-phosphocholine

中图分类号:

O646

王琨琦，张作明*，邢巍*，刘长鹏，周星. 孔蛋白-磷脂仿生膜的葡萄糖氧化酶电化学[J]. 电化学（中英文）, 2014, 20(5): 439-443.

WANG Kun-qi, ZHANG Zuo-ming*, XING Wei*, LIU Chang-peng, ZHOU Xing. Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane[J]. Journal of Electrochemistry, 2014, 20(5): 439-443.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.131176

https://electrochem.xmu.edu.cn/CN/Y2014/V20/I5/439

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孔蛋白-磷脂仿生膜的葡萄糖氧化酶电化学

Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane

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