纳米金三明治结构调制细胞色素c电子传递特性的分子机理研究

doi:10.61558/2993-074X.2913

电化学（中英文） ›› 2012, Vol. 18 ›› Issue (3): 263-268. doi: 10.61558/2993-074X.2913

• 电化学材料基础与表界面研究专辑（中国科学院化学研究所万立骏院士主编） • 上一篇下一篇

纳米金三明治结构调制细胞色素c电子传递特性的分子机理研究

蔺首睿^1,2, 王立旭¹, 姜秀娥^1*, 郭黎平^2*

1. 中国科学院长春应用化学研究所电分析化学国家重点实验室，吉林长春 130022 2. 东北师范大学化学学院，吉林长春 130024

收稿日期:2011-11-29 修回日期:2012-03-21 出版日期:2012-06-28 发布日期:2012-03-25
通讯作者: 姜秀娥, 郭黎平 E-mail:jiangxiue@ciac.jl.cn, guolp078@nenu.edu.cn
基金资助:
吉林省青年基金（No. 201101081）和中科院院长基金资助

On the Molecular Mechanism of Electron Transfer of Cytochrome c Modulated by Gold Nanoparticles in Nano-Sandwich Architecture

LIN Shou-Rui^1,2, WANG Li-Xu¹, JIANG Xiu-E^1*, GUO Li-Ping^2*

1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China； 2. Department of Chemistry, Northeast Normal University, Changchun 130024, China

Received:2011-11-29 Revised:2012-03-21 Published:2012-06-28 Online:2012-03-25
Contact: JIANG Xiu-E, GUO Li-Ping E-mail:jiangxiue@ciac.jl.cn, guolp078@nenu.edu.cn

摘要/Abstract

摘要： 以细胞色素c（Cyt c）为模型蛋白，采用表面增强红外吸收光谱监测了三明治结构所吸附的纳米金对氧化还原诱导的Cyt c表面增强红外差谱的改变. 实验表明，在单层Cyt c分子表面组装金纳米粒子，使得血红素的红外差谱特征峰明显增强，这归因于纳米金和血红素之间的电子传递. 金纳米粒子与Cyt c氧化还原活性中心血红素的相互作用加速了蛋白质的电子传递. 这为实现并优化表面吸附蛋白质的直接电化学提供了一种新技术.

关键词: 细胞色素c, 纳米金, 表面增强红外光谱, 电化学

Abstract: The effects of adsorbed gold nanoparticles on the surface-enhanced infrared absorption (SEIRA) difference spectra of redox-induced cytochrome (Cyt) c in AuNPs/Cyt c/Au sandwich architecture were monitored by SEIRA spectroscopy. The results indicated that the intensity of SEIRA difference spectrum for the vibration of the heme was significantly increased due to the adsorption of gold nanoparticles on the Cyt c. This was induced by the electron transfer between the heme and the gold nanoparticles, which also promoted electron transfer of adsorbed protein. This study suggested a new technique for optimizing the electrochemical property of adsorbed protein.

Key words: Cytochrome c, gold nanoparticles, surface-enhanced infrared absorption spectroscopy, electrochemistry

中图分类号:

O646

蔺首睿, 王立旭, 姜秀娥, 郭黎平. 纳米金三明治结构调制细胞色素c电子传递特性的分子机理研究[J]. 电化学（中英文）, 2012, 18(3): 263-268.

LIN Shou-Rui, WANG Li-Xu, JIANG Xiu-E, GUO Li-Ping. On the Molecular Mechanism of Electron Transfer of Cytochrome c Modulated by Gold Nanoparticles in Nano-Sandwich Architecture[J]. Journal of Electrochemistry, 2012, 18(3): 263-268.

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https://electrochem.xmu.edu.cn/CN/Y2012/V18/I3/263

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纳米金三明治结构调制细胞色素c电子传递特性的分子机理研究

On the Molecular Mechanism of Electron Transfer of Cytochrome c Modulated by Gold Nanoparticles in Nano-Sandwich Architecture

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