表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用

doi:10.13208/j.electrochem.140437

电化学（中英文） ›› 2014, Vol. 20 ›› Issue (6): 521-526. doi: 10.13208/j.electrochem.140437

• 生物电分析化学近期研究专辑（南京大学夏兴华教授主编） • 上一篇下一篇

表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用

黄春芳^1,2，姚桂红¹，邱建丁^1*

1. 南昌大学化学系，江西南昌 330031； 2. 井冈山大学化学化工学院，江西吉安 343009

收稿日期:2014-06-03 修回日期:2014-07-21 出版日期:2014-12-28 发布日期:2014-07-26
通讯作者: 邱建丁 E-mail:jdqiu@ncu.edu.cn
基金资助:
国家自然科学基金项目（Nos. 21163014, 21265017）资助

Preparation of Surface Molecularly Imprinted Magnetic Nanoparticles for Hemoglobin Sensing

HUANG Chun-fang^1,2, YAO Gui-hong¹, QIU Jian-ding^1*

1. Department of Chemistry, Nanchang University, Nanchang 330031, China; 2. School of Chemistry and Chemical Engineering, Jinggangshan University, Ji’an 343009, Jiangxi, China

Received:2014-06-03 Revised:2014-07-21 Published:2014-12-28 Online:2014-07-26
Contact: QIU Jian-ding E-mail:jdqiu@ncu.edu.cn

摘要/Abstract

摘要： 以Fe₃O₄磁性纳米粒子为载体、多巴胺（DA）为功能单体、血红蛋白（Hb）为模板分子，用氯铂酸氧化DA生成聚多巴胺（PDA），同时氯铂酸还原为铂纳米粒子（PtNPs），与Hb一起负载于Fe₃O₄纳米粒子表面，洗脱Hb后合成了表面分子印迹磁性纳米粒子（印迹Fe₃O₄/PDA-PtNPs）. 将印迹Fe₃O₄/PDA-PtNPs修饰于磁性玻碳基底表面，制得印迹Fe₃O₄/PDA-PtNPs修饰电极. 实验结果表明，印迹Fe₃O₄/PDA-PtNPs具有良好的水溶性，粒径分布均匀，生成的PtNPs具有良好的导电性和刚性. 用印迹Fe₃O₄/PDA-PtNPs构建的传感器对Hb具有良好的识别性，在0.14 ~ 2.7 μg·mL^-1 Hb浓度范围与交流阻抗变化值呈良好的线性关系，检出限（S/N=3）为0.05 μg·mL^-1.

关键词: 分子印迹聚合物, 磁性纳米粒子, 多巴胺, 血红蛋白, 电化学传感器

Abstract: Surface molecularly imprinted magnetic nanoparticles (NPs) were prepared by employing dopamine (DA) as a functional monomer, hemoglobin (Hb) as a template, H₂PtCl₆ as an oxidant triggered DA polymerization on the surface of the Fe₃O₄ NPs. The Hb and the formed platinum nanoparticles (PtNPs) were embedded into the polydopamine (PDA). After removal of the Hb, the surface molecularly imprinted magnetic nanoparticles (imprinted Fe₃O₄/PDA-PtNPs) were formed, and then simply immobilized on the magnetic glassy carbon electrode (imprinted Fe₃O₄/PDA-PtNPs/MGCE) for Hb sensing. The imprinted Fe₃O₄/PDA-PtNPs were demonstrated magnetic spherical shape with good solubility in water and narrow size distribution. The PtNPs embedded in the imprinted coating could effectively promote the conductivity and the mechanical strength of imprinted cavity. In addition, the imprinted Fe₃O₄/PDA-PtNPs/MGCE could selectively recognize Hb and was used to detect Hb in the range of 0.14 ~ 2.7 μg?mL^-1 with a detection limit of 0.05 μg?mL^-1 (S/N=3).

Key words: molecularly imprinted polymer, magnetic nanoparticles, dopamine, hemoglobin, electrochemical sensor

中图分类号:

O657.1

黄春芳，姚桂红，邱建丁*. 表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用[J]. 电化学（中英文）, 2014, 20(6): 521-526.

HUANG Chun-fang, YAO Gui-hong, QIU Jian-ding*. Preparation of Surface Molecularly Imprinted Magnetic Nanoparticles for Hemoglobin Sensing[J]. Journal of Electrochemistry, 2014, 20(6): 521-526.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.140437

https://electrochem.xmu.edu.cn/CN/Y2014/V20/I6/521

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表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用

Preparation of Surface Molecularly Imprinted Magnetic Nanoparticles for Hemoglobin Sensing

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