快捷两步法制备金纳米电极用于活体多巴胺检测

doi:10.13208/j.electrochem.181042

电化学（中英文） ›› 2019, Vol. 25 ›› Issue (2): 244-251. doi: 10.13208/j.electrochem.181042

快捷两步法制备金纳米电极用于活体多巴胺检测

关利浩，王超，张望，蔡雨露，李凯^*，林雨青^*

首都师范大学化学系，北京 100048

收稿日期:2018-11-20 修回日期:2018-12-23 出版日期:2019-04-28 发布日期:2018-12-28
通讯作者: 李凯，林雨青 E-mail:linyuqing@cnu.edu.cn; LK123LK@126.com

A Facile Strategy for Two-Step Fabrication of Gold Nanoelectrode for in Vivo Dopamine Detection

GUAN Li-hao, WANG Chao, ZHANG Wang, CAI Yu-lu, LI Kai^*, LIN Yu-qing^*

Department of Chemistry, Capital Normal University, Beijing, China, 100048

Received:2018-11-20 Revised:2018-12-23 Published:2019-04-28 Online:2018-12-28
Contact: LI Kai, LIN Yu-qing E-mail:linyuqing@cnu.edu.cn; LK123LK@126.com
Supported by:
This work was financially supported by National Natural Science Foundation (21575090), High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan (CIT&TCD20190330), Scientific Research Project of Beijing Educational Committee (KM201810028008), Youth Innovative Research Team of Capital Normal University and Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds (19530050179, 025185305000/195).

摘要/Abstract

摘要： 用微电极进行活体检测神经化学物质属于侵入式分析，会对脑组织产生不可避免的损伤，进而在生理上产生一些信号干扰检测过程. 减小电极的尺寸对于减小对脑组织的损伤非常重要. 该研究报道了一种新型制备金纳米电极的方法并将其用于活体鼠脑内多巴胺分析研究. 这种金纳米电极的制备过程包含两步：1）通过离子溅射在毛细管的尖端覆盖一层金种子；2）把覆盖有金种子的毛细管浸入氯金酸和盐酸羟胺混合溶液中湿法沉积生成连续导电金膜. 制备好的纳米电极尖端约300 ~ 400 nm. 该金纳米电极可以应用于多巴胺的检测，并且在多巴胺浓度1.0 ~ 56.0 μmol·L^-1范围内有很好的线性响应，最低检测限低至0.14 μmol·L^-1（信噪比=3）. 该金纳米电极具有优异的电化学性能，可以成功的应用于检测鼠脑纹状体儿茶酚胺的释放.

关键词: 离子溅射, 化学沉积, 金纳米电极, 活体分析, 多巴胺

Abstract: In vivo monitoring neurochemicals with microelectrode is invasive and the damage to brain tissue may inevitably cause disturbance signals physiologically to the measurement. It is of great importance to reduce the electrode size and to decrease the damage. This study demonstrates a novel nanoelectrode preparation methodology for in vivo monitoring dopamine (DA) fluctuation in the living brain of rats with high dependability. The fabrication process of the gold nanoelectrode involving a few minutes consists of only two steps: 1) growing gold nanoseeds on surface of tip of glassy capillary by ion sputtering; 2) wet depositing a continuous conductive gold film composed of gold nanoparticles by dipping the capillary with gold nanoseeds into a freshly mixed chloroauric acid and hydroxylamine hydrochloride for one minute. The tip size of the well-prepared gold nanoelectrode was 300 ~ 400 nanometers. The gold nanoelectrode was able to detect DA and showed a good linearity with the concentration of DA ranging from 1.0 to 56.0 μmol·L^-1 with a limit of detection as low as 0.14 μmol·L^-1 (S/N=3). Benefiting from the excellent electrochemical performance, the gold nanoelectrode was successfully employed for catecholamine release in striatum of living rat brain.

Key words: ion sputtering, wet deposition, gold nanoelectrode, in vivo, dopamine

中图分类号:

O646

关利浩, 王超, 张望, 蔡雨露, 李凯, 林雨青. 快捷两步法制备金纳米电极用于活体多巴胺检测[J]. 电化学（中英文）, 2019, 25(2): 244-251.

GUAN Li-hao, WANG Chao, ZHANG Wang, CAI Yu-lu, LI Kai, LIN Yu-qing. A Facile Strategy for Two-Step Fabrication of Gold Nanoelectrode for in Vivo Dopamine Detection[J]. Journal of Electrochemistry, 2019, 25(2): 244-251.

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https://electrochem.xmu.edu.cn/CN/Y2019/V25/I2/244

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快捷两步法制备金纳米电极用于活体多巴胺检测

A Facile Strategy for Two-Step Fabrication of Gold Nanoelectrode for in Vivo Dopamine Detection

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