关键词: 对羟基苯硼酸, 金纳米粒子, 过氧化氢, 电化学检测

Abstract:

We developed a new method for electrochemical detection of hydrogen peroxide (H₂O₂) based on boronate oxidation of p-hydroxyphenylboronic acid. This method using p-aminophenol which is produced from the reaction of H₂O₂ and p-aminophenylboronic acid as a well electrochemical probe, combined with a gold nanoparticles (AuNPs) modified electrode for an indirect detection of H₂O₂. Because of the large surface area and enhanced electrocatalytic behavior by the AuNPs modified electrode, the detection sensitivity was improved. The method could detect H₂O₂ in the concentration range of 1 ~ 100 μmol•L^-1 and 0.1 ~ 1 mmol•L^-1 in 0.1 mol•L^-1 pH 7.5 PBS containing 1.0 mmol•L^-1 p-hydroxyphenylboronic acid. The low detection limit was 0.5 μmol•L^-1. The proposed method had good selectivity and stability. Moreover, the method was quick, simple and cheap, which has potential application in real samples analysis.

Key words: p-Hydroxyphenylboronic acid, hydrogen peroxide, gold nanoparticles, electrochemical detection