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电化学(中英文) ›› 2023, Vol. 29 ›› Issue (6): 2218002.  doi: 10.13208/j.electrochem.2218002

所属专题: “电分析”专题文章

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基于电化学阻抗谱的致病菌检测传感器的研究进展

陈涛a, 许元红a,*(), 李景虹b,*()   

  1. a青岛大学生命科学学院、生物医学工程研究院,山东 青岛 266071
    b清华大学化学系,生命有机磷化学及化学生物学教育部重点实验室,北京 100084
  • 收稿日期:2022-10-31 修回日期:2023-01-05 接受日期:2023-05-17 出版日期:2023-06-28 发布日期:2023-05-24

Recent Advances in Electrochemical Impedance Spectroscopy-Based Pathogenic Bacteria Sensing

Tao Chena, Yuan-Hong Xua,*(), Jing-Hong Lib,*()   

  1. aInstitute of Biomedical Engineering, College of Life Sciences, Qingdao University, Qingdao 266071, China
    bDepartment of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China
  • Received:2022-10-31 Revised:2023-01-05 Accepted:2023-05-17 Published:2023-06-28 Online:2023-05-24
  • Contact: *Tel: (86-532)85956199, E-mail: yhxu@qdu.edu.cn (Y. Xu); Tel: (86-10)62795290, E-mail: jhli@mail.tsinghua.edu.cn (J. Li)

摘要:

几千年来,致病菌对人类健康构成了巨大威胁。实现致病菌的实时监测可有效阻止致病菌的传播,从而降低对人类健康的威胁。迄今为止,已有电化学、光学、压电和量热等多种技术用于细菌的检测。其中,基于电化学阻抗技术的传感器由于其成本低、读取时间短、重现性好、设备便携等优点,在实时细菌检测中展现出了巨大的应用潜力。本文主要综述了近三年来电化学阻抗技术在细菌传感中的典型应用。众所周知,电极材料在基于电化学阻抗的传感器的构建中发挥着极其重要的作用,因为细菌生物识别元件的固定化,以及所制备的传感器的灵敏度、经济性和便携性都主要取决于电极材料。因此,为了向新入行的研究人员提供基于不同电极材料制备电化学阻抗传感器清晰的制备过程,我们尝试根据不同的电极平台对基于电化学阻抗技术的传感器进行分类。此外,还讨论了目前的难点、未来的应用方向和前景。我们希望通过本文的综述,能够为刚进入该领域的研究人员开展基于电化学阻抗技术,制备快速、灵敏、准确地检测多种致病菌的传感器研究提供指导。

关键词: 电化学阻抗谱, 致病菌检测, 生物识别元件, 电极材料

Abstract:

Pathogenic bacteria have been throwing great threat on human health for thousands of years. Their real-time monitoring is in urgent need as it could effectively halt the spread of pathogenic bacteria and thus reducing the risk to human health. Up till now, diverse technologies such as electrochemistry, optics, piezoelectricity and calorimetry have been developed for bacteria sensing. Therein, electrochemical impedance spectroscopy (EIS)-based sensors show great potential in point-of-care bacterial analysis because of their low-cost, short read-out time, good reproducibility, and portable equipment construction. In this review, we will primarily summarize the typical applications of electrochemical impedance technology in bacteria sensing based on different electrodes in the last three years. As we know, the electrode materials play an extremely important role in the construction of EIS-based sensors because not only the immobilization of bio-recognition elements for bacteria, but also the sensitivity, economical efficiency and portability of the as-prepared sensors are mainly determined by the electrode materials. Therefore, in order to provide new researchers a clear preparation process for EIS-based sensors fabricated with different electrodes, we try to classify the EIS-based sensors according to the different electrode platforms. Moreover, present difficulties, future directions and perspectives for their applications are also discussed. It can provide guidance in future study of novel EIS-based sensors for rapid, sensitive and accurate sensing of diverse pathogenic bacteria.

Key words: electrochemical impedance spectroscopy, pathogenic bacteria detection, bio-recognition elements, electrode material