基于聚酚藏花红功能碳纳米管生物阳极的制备及其在乙醇传感中的应用研究(英文)

doi:10.61558/2993-074X.2093

电化学（中英文） ›› 2011, Vol. 17 ›› Issue (3): 263-270. doi: 10.61558/2993-074X.2093

• 化学-生物传感技术近期研究专辑（上海师范大学章宗穰教授主编） • 上一篇下一篇

基于聚酚藏花红功能碳纳米管生物阳极的制备及其在乙醇传感中的应用研究(英文)

Farhana S. Saleh1, Takeyoshi Okajima (岡島武義) 1, Lanqun Mao (毛兰群）2,Takeo Ohsaka（大坂武男）1*

1. 东京工业大学电子化学系, 日本, 东京226-8502; 2. 北京分子科学国家实验室, 中国科学院活体分析化学重点实验室, 北京 100190

收稿日期:2011-06-07 修回日期:2011-07-11 出版日期:2011-08-28 发布日期:2011-07-20
通讯作者: Takeo Ohsaka E-mail:ohsaka@echem.titech.ac.jp
基金资助:
The present work was financially supported by Grant-in-Aid for Scientific Research (A) (No. 19206079) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, Tokyo Institute of Technology Global COE Program for Energy Science, and Japan-China Research Program on Enzyme-based Biofuel Cells organized and sponsored by Japan Science and Technology (JST) and Natural Science Foundation of China (NSFC).

Development of dehydrogenase-based bioanode using poly(phenosafranin)-functionalized SWCNT nanocomposites and its application to ethanol biosensor

Farhana S. Saleh1, Takeyoshi Okajima1, Lanqun Mao2, and Takeo Ohsaka1*

1. Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan; 2. Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, the Chinese Academy of Sciences(CAS), Beijing 100190, China

Received:2011-06-07 Revised:2011-07-11 Published:2011-08-28 Online:2011-07-20
Contact: Takeo Ohsaka E-mail:ohsaka@echem.titech.ac.jp
Supported by:
The present work was financially supported by Grant-in-Aid for Scientific Research (A) (No. 19206079) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, Tokyo Institute of Technology Global COE Program for Energy Science, and Japan-China Research Program on Enzyme-based Biofuel Cells organized and sponsored by Japan Science and Technology (JST) and Natural Science Foundation of China (NSFC).

摘要/Abstract

摘要： 本文利用电化学聚合酚藏花红（PPS）功能化单壁碳纳米管作为烟酰胺辅酶（NADH）氧化的电化学催化剂，研制了基于乙醇脱氢酶的安培型乙醇生物电化学传感器。PPS通过在酚藏花红的溶液电化学聚合得到，在0.0 V时对NADH具有很好的催化性能。单体酚藏花红则由于电化学电位太低（-0.48 V）而对NADH没有催化性能。所制备的传感器的性能通过循环伏安和计时安培法来表征。实验结果表明：碳纳米管的载量，固定化酶的量，NAD+的浓度以及溶液的pH都直接影响传感器的性能。在优化的条件下，所制备的乙醇传感器在0.2 V的电位下，对乙醇响应的灵敏度为2.0 μA cm-2 mM–1，检测限为36 μM。所制备的传感器具有很好的稳定性，连续测定45 min后响应电流下降值只是起始响应值的7%。本文的研究为电化学生物传感器的研究提供了新的思路

关键词: 酚藏花红（PS）, 电化学聚合, 烟酰胺辅酶（NADH）, 单壁碳纳米管（SWCNT), 电催化

Abstract: A New type of dehydrogenase-based amperometric ethanol biosensor was constructed using alcohol dehydrogenase (ADH) which was immobilized on the edge-plane pyrolytic graphite (EPPG) electrode modified with poly(phenosafranin)-functionalized single-walled carbon nanotube (PPS-SWCNT). The PPS-SWCNT modified EPPG electrode was prepared by electropolymerization of phenosafranin on the EPPG electrode which was previously coated with SWCNT. The performance of the ADH/PPS-SWCNT/EPPG electrode was evaluated using cyclic voltammetry and amperometry in the presence of ethanol. The fabricated ethanol biosensor provided a reasonable sensitivity of 2.0 μA cm–2 mM–1 and a low detection limit (36 μM) for the electrocatalytic oxidation of ethanol with a linear concentration dependence upto ~ 1.0 mM at a detection potential of 0.2 V.

Key words: Phenosafranin, electropolymerization, NADH, SWCNT, electrocatalysis

中图分类号:

O646
TP212.3

Farhana S. Saleh, Takeyoshi Okajima, Lanqun Mao, Takeo Ohsaka. 基于聚酚藏花红功能碳纳米管生物阳极的制备及其在乙醇传感中的应用研究(英文)[J]. 电化学（中英文）, 2011, 17(3): 263-270.

Farhana S. Saleh, Takeyoshi Okajima, Lanqun Mao, Takeo Ohsaka. Development of dehydrogenase-based bioanode using poly(phenosafranin)-functionalized SWCNT nanocomposites and its application to ethanol biosensor[J]. Journal of Electrochemistry, 2011, 17(3): 263-270.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.61558/2993-074X.2093

https://electrochem.xmu.edu.cn/CN/Y2011/V17/I3/263

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基于聚酚藏花红功能碳纳米管生物阳极的制备及其在乙醇传感中的应用研究(英文)

Development of dehydrogenase-based bioanode using poly(phenosafranin)-functionalized SWCNT nanocomposites and its application to ethanol biosensor

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