基于碳纳米管的高灵敏度免标记电化学核酸适体传感电极

doi:10.13208/j.electrochem.131161

电化学（中英文） ›› 2014, Vol. 20 ›› Issue (4): 386-391. doi: 10.13208/j.electrochem.131161

• 电化学近期研究专辑（厦门大学姜艳霞教授主编） • 上一篇下一篇

基于碳纳米管的高灵敏度免标记电化学核酸适体传感电极

邓春艳^1,2，范慧敏²，向娟²，李元建^1*

1. 中南大学药学院，湖南长沙 410083；2. 中南大学化学化工学院，湖南长沙 410083

收稿日期:2013-11-26 修回日期:2014-04-09 出版日期:2014-08-28 发布日期:2014-04-15
通讯作者: 李元建 E-mail:yuan_jianli@yahoo.com
基金资助:
国家自然科学基金项目（No. 21005090）、中国博士后基金项目（No. 2012M510136）和中国博士基金特别资助项目（No. 2013T60774）

A Sensitive and Label-Free Electrochemical Aptasensor based on Signal Amplification of Carbon Nanotubes

DENG Chun-yan^1,2, FAN Hui-min², XIANG Juan², LI Yuan-jian^1*

1. College of Pharmacy, Central South University, Changsha 410083, China; 2. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Received:2013-11-26 Revised:2014-04-09 Published:2014-08-28 Online:2014-04-15
Contact: LI Yuan-jian E-mail:yuan_jianli@yahoo.com

摘要/Abstract

摘要： 以电活性钌化合物[Ru(NH₃)₆]³⁺为信号传感源，借助碳纳米管构建了高灵敏检测腺苷免标记电化学传感电极（BSA/Apt/CNTs/GC）. BSA/Apt/CNTs/GC电极在最佳实验条件下检测腺苷线性范围为5.0×10^-11 ~ 1.0×10^-7 mol·L^-1，检测下限为2.7×10^-11 mol·L^-1. 该传感电极有较高的灵敏度、良好的选择性、重现性和稳定性. 与传统标记型适体传感电极相比，其制作简便，也许还适用于其他小分子和蛋白质的检测，有一定的普适性.

关键词: 核酸适体, 腺苷, 免标记, 碳纳米管

Abstract: A label-free electrochemical sensing electrode for highly sensitive detection of adenosine was constructed based on the signal amplification of carbon nanotubes (CNTs). The change in the interfacial feature of the modified electrode was characterized by electrochemical impedance spectroscopy. Using [Ru(NH₃)₆]³⁺ as the signaling moiety, adenosine with concentrations as low as 0.027 nmol·L^-1 can be selectively detected. Additionally, the fabrication of this present aptasensor was simple, time-saving and cost-effective. Compared with other reported aptasensors, the proposed aptasensor had advantages of excellent sensitivity, selectivity and simplicity, which plays a potential role in development of aptasensor.

Key words: carbon nanotubes, aptamer, label-free, adenosine

中图分类号:

O646

邓春艳，范慧敏，向娟，李元建*. 基于碳纳米管的高灵敏度免标记电化学核酸适体传感电极[J]. 电化学（中英文）, 2014, 20(4): 386-391.

DENG Chun-yan, FAN Hui-min, XIANG Juan, LI Yuan-jian*. A Sensitive and Label-Free Electrochemical Aptasensor based on Signal Amplification of Carbon Nanotubes[J]. Journal of Electrochemistry, 2014, 20(4): 386-391.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.131161

https://electrochem.xmu.edu.cn/CN/Y2014/V20/I4/386

参考文献

[1] McMillan M R, Burnstock G, Haworth S G. Vasodilatation of intrapulmonary arteries to P2-receptor nucleotides in normal and pulmonary hypertensive newborn piglets[J]. British Journal of Pharmacology, 1999, 128(3): 543-548.
[2] Wu Z S, Guo M M, Zhang S B, et al. Reusable electrochemical sensing platform for highly sensitive detection of small molecules based on structure-switching signaling aptamers[J]. Analytical Chemistry, 2007, 79(7): 2933-2939.
[3] Bennett H J, White T D, Semba K. Activation of metabotropic glutamate receptors increases extracellular adenosine in vivo[J]. NeuroReport, 2000, 11(16): 3489-3492.
[4] Craig C G, White T D. NMDA-evoked adenosine release from rat cortex does not require the intermediate formation of nitric oxide[J]. Neuroscience Letters, 1993, 158(2): 167-169.
[5] Wilson D S, Szostak J W. In vitro selection of functional nucleic acids[J]. Annual Review Biochemistry, 1999, 68: 611-647.
[6] Liu J, Cao Z, Lu Y. Functional nucleic acid sensors[J]. Angewandte Chemie International Edition, 2009, 109(5): 1948-1998.
[7] Wang J, Jiang Y, Zhou C, et al. Aptamer-based ATP assay using a luminescent light switching complex[J]. Analytical Chemistry, 2005, 77(11): 3542-3546.
[8] Xiao Y, Heeger A J, Plaxco K W, et al. A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement[J]. Journal of the American Chemical Society, 2005, 127(51): 17990-17991.
[9] Radi A E, Acero Sánchez J L, Baldrich E, et al. Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor[J]. Journal of the American Chemical Society, 2006, 128(1): 117-124.
[10] Urata H, Nomura K, Akagi M, et al. Fluorescent-labeled single-strand ATP aptamer DNA: Chemo- and enantio-selectivity in sensing adenosine[J]. Biochemical and Biophysical Research Communications, 2007, 360(2): 459-463.
[11] Chen J W, Liu X P, Feng K J, et al. Detection of adenosine using surface-enhanced Raman scattering based on structures-witching signaling aptamer[J]. Biosensors and Bioelectronics, 2008, 24(1): 66-71.
[12] Ho D, Falter K, Severin P, et al. DNA as a force sensor in an aptamerbased biochip for adenosine[J]. Analytical Chemistry, 2009, 81(8): 3159-3164.
[13] Zheng F, Wu Z S, Zhang S B, et al. Aptamer based electrochemical biosensors for highly selective and quantitative detection of adenosine[J]. Chemical Research in Chinese Universities, 2008, 24(2): 138-142.
[14] Deng Q, Watson C J, Kennedy R T. A ptamer affinity chromatography for rapid assay of adenosine in microdialysis samples collected in vivo[J]. Journal of Chromatography A, 2003, 1005(1/2): 123-130.
[15] Zhou L, Ou L J, Chu X, et al. Aptamer-based rolling circle amplification: A platform for electrochemical detection of protein[J]. Analytical Chemistry, 2007, 79(19): 7492-7500.
[16] Shlyahovsky B, Li D, Weixmann Y. Splotlighting of cocaine by an autonomous aptamer-based machine[J]. Journal of the American Chemical Society, 2007, 129(13): 3814-3815.
[17] Weizmann Y, Beissenhirtz M K, Cheglakov Z, et al. A virus spotlighted by an autonoumous DNA machine[J]. Angewandte Chemie International Edition, 2006, 45(44): 7384-7388.
[18] Li B L, Wang Y L, Wei H, et al. Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model[J]. Biosensors and Bioelectronics, 2007, 23(7): 965-970.
[19] Xu D K, Xu, D W, Yu X B, et al. Label-free electrochemical detection for aptamer-based array electrodes[J]. Analytical Chemistry, 2005, 77(16): 5107-5113.
[20] Wang D Y, Lai B Y, Sen D. A general strategy for effector-mediated control of RNA-cleaving ribozymes and DNA enzymes[J]. Journal of Molecular Biology, 2002, 318(1): 33-43.
[21] Zhao W, Ali M M, Aguirre S D, et al. Paper-based bioassays using gold nanoparticle Colorimetric probe[J]. Analytical Chemistry, 2008, 80(22):8431-8437.
[22] Liu J, Lu Y. Adenosine-dependent assembly of aptazyme-functionalized gold nanoparticles and its application as a colorimetric biosensor[J]. Analytical Chemistry, 2004, 76(6): 1627-1632.
[23] Wang J L, Munir A, Zhu Z Z, et al. Magnetic nanoparticle enhanced surface plasmon resonance sensing and its application for the ultrasensitive detection of magnetic nanoparticle-enriched small molecules[J]. Analytical Chemistry, 2010, 82(16): 6782- 6789.
[24] Feng K J, Sun C H, Kang Y, et al. Label-free electrochemical detection of nanomolar adenosine based on target-induced aptamer displacement[J]. Electrochemistry Communications, 2008, 10(4): 531-535.
[25] Chen J W, Liu X P, Feng K J. Detection of adenosine using surface-enhanced Raman scattering based on structure-switching signaling aptamer[J]. Biosensors and Bioelectronics, 2008, 24(1): 66-71.
[26] Zhao W J, Chiuman W, Lam J C F, et al. DNA aptamer folding on gold nanoparticles: From colloid chemistry to biosensors[J]. Journal of the American Chemical Society, 2008, 130(11): 3610-3618.

基于碳纳米管的高灵敏度免标记电化学核酸适体传感电极

A Sensitive and Label-Free Electrochemical Aptasensor based on Signal Amplification of Carbon Nanotubes

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

[1]	高梦婷, 卫莹, 霍雪萌, 朱文洁, 刘箐箐, 强晋源, 刘婉婉, 王颖, 李旭, 黄剑锋, 冯永强. 氮掺杂碳纳米管上钴和钌位点之间的电子通信促进碱性析氢反应[J]. 电化学（中英文）, 2024, 30(9): 2403081-.
[2]	李家俊, 张伟彬, 刘鑫宇, 杨静蕾, 尹易, 杨泽钦, 马雪婧. 二硫化钼和碳纳米管复合物电极用于盐差能转换[J]. 电化学（中英文）, 2024, 30(6): 2307121-.
[3]	刘芳艳, 张倩, 李玥琨, 黄丰, 王梦晔. Co_1-_xS-MnS@CNTs/CNFs的制备及其氧还原电催化性能[J]. 电化学（中英文）, 2021, 27(3): 301-310.
[4]	李二岭, 杨发, 阮明波, 宋平, 徐维林. Fe-N共掺杂纳米碳材料的形貌对电化学还原反应的影响[J]. 电化学（中英文）, 2019, 25(4): 486-496.
[5]	张雅琳，陈驰，邹亮亮，邹志青，杨辉. Fe-N共掺杂的碳纳米管串联空心球对氧还原反应的电催化[J]. 电化学（中英文）, 2018, 24(6): 726-732.
[6]	余林颇, 陈政. 提高电容性电化学储能装置能量容量的一些尝试[J]. 电化学（中英文）, 2017, 23(5): 533-547.
[7]	陈姿颖，武倩倩，张健青，朱英红，马淳安. 负载型离子液体在C-H键电氧化活化中的应用研究[J]. 电化学（中英文）, 2017, 23(1): 1-6.
[8]	彭花萍, 余美玲, 刘馨, 刘盼, 陈伟, 刘爱林, 林新华*. 金纳米粒子/聚多巴胺/碳纳米管修饰玻碳电极对核黄素的测定[J]. 电化学（中英文）, 2016, 22(1): 43-48.
[9]	廉锋, 辛永磊, 马伯江, 许立坤. 添加碳纳米管对钌铱锡氧化物阳极性能的影响[J]. 电化学（中英文）, 2015, 21(4): 375-381.
[10]	郝亮，申来法，王婕，朱佳佳，赵笑晨，张校刚*. 碳纳米管/五氧化二钒空心球的制备及其电化学性能研究[J]. 电化学（中英文）, 2015, 21(2): 152-156.
[11]	严乙铭,毛兰群*. 全酶型乙醇/氧气生物燃料电池的构筑及性能研究[J]. 电化学（中英文）, 2015, 21(1): 8-12.
[12]	张云，胡劲松，江文杰，郭琳，魏子栋，万立骏. 通过铁掺杂和造孔提高氮掺杂石墨烯/碳纳米管复合物电催化氧还原性能的研究（英文）[J]. 电化学（中英文）, 2014, 20(5): 401-409.
[13]	范丽丽，武丽娜，屈志宇，刘丹凤，张俊明，樊思明，樊友军*. Pt/DNA-MWCNTs/GC电极制备及其H₂O₂还原的电催化活性[J]. 电化学（中英文）, 2014, 20(5): 459-464.
[14]	杜学萍，娄童芳，董莹，屈建莹*. 聚茜素红-多壁碳纳米管复合膜修饰电极的研制及叶酸含量测定[J]. 电化学（中英文）, 2014, 20(5): 482-485.
[15]	彭花萍，查代君，黄郑隽，陈伟，刘爱林，林新华. 二茂铁功能化Fe₃O₄/碳纳米管/壳聚糖复合膜葡萄糖生物传感电极的研究[J]. 电化学（中英文）, 2014, 20(1): 33-38.