关键词: 适配体, 妥布霉素, 电化学分析, 金纳米粒子

Abstract: A novel well-constructed electrochemical aptamer-based sensor for the detection of tobramycin was presented, using differential pulse voltammetry (DPV) as a detection technique and methylene blue（MB） as an electrochemical indicator. A glassy carbon electrode modified with a nanocomposite of Au nanoparticles/polyaniline/titania nanotubes (AuNPs/PANI/TNTs) was constructed as the electrode scaffold. The nanocomposite was characterized by transmission electron microscopy and X-ray photoelectron spectroscopy in detail. The results of cyclic voltammetry and electrochemical impedance measurements demonstrated that the AuNPs/PANI/TNTs nanocomposites can improve greatly the electron transfer on the interface. For the detection of tobramycin, the DPV results showed a linear relationship between the current response and the concentration of tobramycin, and a wide range of detection from 0.5 μmol·L^-1 to 70 μmol·L^-1. The presented aptamer-based biosensor exhibited excellent sensitivity and reproducibility, which would have a potential application in bioanalysis and clinical diagnostics.

Key words: aptasensor, tobramycin, electrochemical analysis, Au nanoparticles