血红蛋白在类生物膜修饰电极上的直接电化学

doi:10.61558/2993-074X.1902

电化学（中英文） ›› 2008, Vol. 14 ›› Issue (3): 258-262. doi: 10.61558/2993-074X.1902

血红蛋白在类生物膜修饰电极上的直接电化学

后雯璟;周琳;吴霞琴;张红霞;谢文;杨柳;陆中庆;

上海师范大学生命与环境科学学院化学系;

收稿日期:2008-08-28 修回日期:2008-08-28 出版日期:2008-08-28 发布日期:2008-08-28

Direct Electrochemistry of Hemoglobin on the Electrode Modified with DDAB and SWCNT

HOU Wen-jing,ZHOU Lin,WU Xia-qin,ZHANG Hong-xia,XIE Wen,YANG Liu,LU Zhong-qing

(Department of Chemistry,Shanghai Normal University,Shanghai 200234,China

Received:2008-08-28 Revised:2008-08-28 Published:2008-08-28 Online:2008-08-28

摘要/Abstract

摘要： 使用掺合单壁碳纳米管(SWCNT)的不溶性表面活性剂双十二烷基二甲基溴化铵(DDAB)修饰玻碳电极,并将血红蛋白(Hb)固定在修饰膜中制得了稳定的固载Hb的修饰电极.循环伏安和交流阻抗测试表明,固定在电极上的Hb是一个受吸附控制的可逆电子传递过程.该氧化还原过程的CV扫描峰电位与溶液pH值成良好的线性关系,斜率39 mV/pH,表明在发生Hb直接电子传递反应的同时伴随有质子参与反应.掺合SWCNT的类生物膜修饰电极较之不掺合SWCNT的修饰电极对氧气的还原具有更好的催化作用.并以SWCNT掺合量为1 mg.mL-1的DDAB修饰电极性能最佳.

关键词: 血红蛋白, 碳纳米管, DDAB, 直接电子传递, 循环伏安, 电化学交流阻抗谱

Abstract: Hemoglobin(Hb) was immobilized onto the surface of glassy carbon electrode with the single-wall carbon nanotubes and surfactant didodecyldimethylamonium bromide(DDAB) modified previously and its electrochemical behaviors have been investigated by cyclic voltammetry.A pair of well-defined redox peaks has been obtained.Cyclic voltammetry and electrochemical impedance spectroscopy(EIS) were also used to study on the effect of different concentration of SWCNT in DDAB.The result shows that the 1 mg/mL is the best choice.The dependence of E0' on solution pH with the slope of 39 mV/pH,indicated that redox of the Hb is a one electron reaction with two protons transfer.The results also showed that the immobilized Hb keeped its electrocatalytic activity toward the reduction of O2.

Key words: hemoglobin(Hb), single-wall carbon nano-tube(SWCNT), didodecyldimethylamonium bromide(DDAB), direct electron transfer, cyclic voltammetry, electrochemical impedance spectroscopy

中图分类号:

R341

后雯璟, 周琳, 吴霞琴, 张红霞, 谢文, 杨柳, 陆中庆, . 血红蛋白在类生物膜修饰电极上的直接电化学[J]. 电化学（中英文）, 2008, 14(3): 258-262.

HOU Wen-jing, ZHOU Lin, WU Xia-qin, ZHANG Hong-xia, XIE Wen, YANG Liu, LU Zhong-qing. Direct Electrochemistry of Hemoglobin on the Electrode Modified with DDAB and SWCNT[J]. Journal of Electrochemistry, 2008, 14(3): 258-262.

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https://electrochem.xmu.edu.cn/CN/Y2008/V14/I3/258

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血红蛋白在类生物膜修饰电极上的直接电化学

Direct Electrochemistry of Hemoglobin on the Electrode Modified with DDAB and SWCNT

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