基于电化学双共价键法构建可再生高灵敏的电化学发光适体传感器检测可卡因

doi:10.13208/j.electrochem.181044

电化学（中英文） ›› 2019, Vol. 25 ›› Issue (2): 223-231. doi: 10.13208/j.electrochem.181044

基于电化学双共价键法构建可再生高灵敏的电化学发光适体传感器检测可卡因

王晓飞^a，张婷^a，王冰，漆红兰^*，张成孝^*

陕西省生命分析化学重点实验室，陕西师范大学化学化工学院，陕西西安 710062

收稿日期:2018-12-04 修回日期:2019-01-15 出版日期:2019-04-28 发布日期:2019-01-29
通讯作者: 漆红兰, 张成孝 E-mail: honglanqi@snnu.edu.cn; cxzhang@snnu.edu.cn
基金资助:
国家自然科学基金项目（No. 21775097）和中央高校基础研究经费（No. GK201801006）资助

Sensitive and Reusable Electrogenerated Chemiluminescence Aptasensor Fabricated by Electrochemically Double Covalent Coupling Method for the Detection of Cocaine

WANG Xiao-fei^a, ZHANG Ting^a, WANG Bing, QI Hong-lan^*, ZHANG Cheng-xiao^*

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, P.R. China

Received:2018-12-04 Revised:2019-01-15 Published:2019-04-28 Online:2019-01-29
Contact: QI Hong-lan, ZHANG Cheng-xiao E-mail: honglanqi@snnu.edu.cn; cxzhang@snnu.edu.cn
Supported by:
We thank the National Science Foundation of China (No. 21775097) and the Fundamental Research Funds from the Central Universities (No. GK201801006)

摘要/Abstract

摘要： 基于点击化学和重氮盐法的双共价键固定化方法，制备了一种高灵敏、可重复使用的电化学发光(ECL)适体传感器. 该方法以可卡因为分析物，以可卡因适体为分子识别物质，以钌联吡啶衍生物为ECL信号物质. 采用电化学方法在玻碳电极表面重氮化叠氮苯胺，通过点击反应连接炔基功能化的钌联吡啶衍生物标记可卡因适体，获得适体传感器. 该传感器在共反应剂存在下，产生弱的电化学发光信号，可卡因存在下，电化学发光信号增加. 基于此，建立了“信号增强”型检测可卡因的电化学发光分析新方法. 电化学发光信号与可卡因浓度在0.1 nmol·L^-1 ~ 100 nmol·L^-1范围内呈良好的线性关系，检出限为60 pmol·L^-1. 该传感器具有良好的稳定性，可重复多次使用. 该双共价键法在构建ECL传感器方面具有很好的应用前景.

关键词: 电化学发光, 适体, 可卡因, 点击化学, 重氮盐法

Abstract: A double covalent coupling method based on click chemistry and diazonium chemistry was developed for the fabrication of a sensitive and reusable electrogenerated chemiluminescence (ECL) aptasensor. As a proof-of-concept, cocaine was chosen as a model target, and a specific aptamer was chosen as a recognition molecule element, while ruthenium complex derivative (Ru1) as an ECL signal compound. The ECL aptasensor was fabricated by electrochemically diazotizing a phenylazide on the surface of a glassy carbon electrode, and then, coupling the alkyne functionalized Ru1-labeled cocaine aptamer through click chemistry. The ECL aptasensor was facilely used to determine cocaine in the range from 0.1 nmol·L^-1 to 100 nmol·L^-1 with the detection limit as low as 60 pmol·L^-1 within 30 min via “signal on” mode. The advantage of the ECL aptasensor was further exemplified by good stability and reusability. This work demonstrates that the double covalent coupling method is a promising approach for the fabrication of the ECL biosensor.

Key words: electrogenerated chemiluminescence, aptasensor, cocaine, click chemistry, diazotization

中图分类号:

O646

王晓飞, 张婷, 王冰, 漆红兰, 张成孝. 基于电化学双共价键法构建可再生高灵敏的电化学发光适体传感器检测可卡因[J]. 电化学（中英文）, 2019, 25(2): 223-231.

WANG Xiao-fei, ZHANG Ting, WANG Bing, QI Hong-lan, ZHANG Cheng-xiao. Sensitive and Reusable Electrogenerated Chemiluminescence Aptasensor Fabricated by Electrochemically Double Covalent Coupling Method for the Detection of Cocaine[J]. Journal of Electrochemistry, 2019, 25(2): 223-231.

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https://electrochem.xmu.edu.cn/CN/Y2019/V25/I2/223

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基于电化学双共价键法构建可再生高灵敏的电化学发光适体传感器检测可卡因

Sensitive and Reusable Electrogenerated Chemiluminescence Aptasensor Fabricated by Electrochemically Double Covalent Coupling Method for the Detection of Cocaine

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