关键词: 光谱电化学, 表面增强红外光谱, 红外光谱电化学, 生物分析

Abstract: Surface-enhanced infrared absorption spectroscopy (SEIRAS), especially in attenuated total reflectance (ATR) mode, taking advantages of surface-enhancement and near-field optical effect of enhancing substrate, is a ultra-sensitive infrared spectroscopy, which could achieve surface-selected detection at a sub-monolayer level. Since the enhancing substrate could simultaneously serve as a working electrode, ATR-SEIRAS is a readily surface-sensitive in situ label-free spectroelectrochemistry technique. With the advantages of small influence from metal species on enhancement effect, good potential reversibility of spectra, simple surface selection rule and sensitivity to polar molecules, ATR-SEIRAS has been widely applied in the fields of orientation analysis and species identification of interfacial adsorbed molecules, interfacial catalytic reaction mechanism elucidation, and interfacial molecular interaction study. In recent years, ATR-SEIRAS has been demonstrated to be a powerful tool in biomolecule analysis, and great progress has been made. Therefore, this review briefly introduces the basic principle and basic technical characteristics of SEIRAS, and then emphasizes the application and development of ATR-SEIRAS in protein structure-function study, artificial biomimetic membrane research, whole cell analysis, as well as electrocatalytic interface probing, through discussing several representative research works. Finally, some perspectives for future development of SEIRAS are discussed.

Key words: spectroelectrochemistry, surface-enhanced infrared absorption spectroscopy, infrared ctroelectrochemistry, bioanalysis