“金标银染”放大技术的羟基自由基灵敏检测

doi:10.13208/j.electrochem.140442

电化学（中英文） ›› 2015, Vol. 21 ›› Issue (1): 22-28. doi: 10.13208/j.electrochem.140442

• 生物电分析化学近期研究专辑（南京大学夏兴华教授主编） • 上一篇下一篇

“金标银染”放大技术的羟基自由基灵敏检测

杨妍^1,2，喻鹏¹，张小华^1*，陈金华^1*

1. 湖南大学化学化工学院，化学生物传感与计量学国家重点实验室，湖南长沙 410082；2. 南阳师范学院化学与制药工程学院，河南南阳 473061

收稿日期:2014-08-04 修回日期:2014-09-25 出版日期:2015-02-28 发布日期:2014-09-30
通讯作者: 陈金华, 张小华 E-mail:chenjinhua@hnu.edu.cn; mickyxie@hnu.edu.cn
基金资助:
国家自然科学基金项目（No. 21275041, No. 21235002, No. 21221003）资助

Sensitive Detection of Hydroxyl Radical based on Silver-Enhanced Gold Nanoparticle Label

YANG Yan^1,2, YU Peng¹, ZHANG Xiao-hua^1,*, CHEN Jin-hua^1,*

1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha Hunan 410082, China; 2. College of Chemistry and Pharmaceutical engineering, Nanyang Normal University, Henan Nanyang, 473061, China

Received:2014-08-04 Revised:2014-09-25 Published:2015-02-28 Online:2014-09-30
Contact: CHEN Jin-hua, ZHANG Xiao-hua E-mail:chenjinhua@hnu.edu.cn; mickyxie@hnu.edu.cn

摘要/Abstract

摘要： 本文采用银染增强金纳米粒子（AuNPs）为信号因子，构建了一种新型的灵敏检测羟基自由基（·OH）的DNA电化学传感器. 首先，巯基化的DNA1通过Au—S键自组装于金基底电极表面. 然后，由Fenton反应产生的·OH可引起电极表面DNA1自组装层的氧化损伤裂解，未损伤的DNA1可与功能化AuNPs上的DNA2杂交. 利用AuNPs对银离子的催化还原反应，将银原子沉积在AuNPs的周围，形成一层银外壳，再用差分脉冲伏安法（DPV）技术对沉积的银进行电化学检测，从而实现·OH的定量分析. 研究结果表明, 在最优实验条件下，该传感器检测·OH的线性范围为0.2 ~ 200 μmol·L^-1，检测下限为50 nmol·L^-1. 该传感器有较好的重复性、选择性，并在抗氧化剂抗氧化能力评估方面具有潜在应用价值.

关键词: 羟基自由基, DNA损伤, 金纳米粒子, 银染增强, 电化学传感器

Abstract: A novel DNA-based electrochemical sensor has been successfully constructed for sensitive detection of hydroxyl radical (·OH) based on the silver-enhanced gold nanoparticle label. Thiolated DNA1 was firstly immobilized on the gold electrode through Au—S bonds. The ·OH generated from Fenton reaction could induce serious oxidative damage of the DNA1 layer on the electrode surface. Then DNA2-functionalized gold nanoparticles (DNA2-AuNPs) were linked on the electrode through the hybridization between DNA2 and undamaged DNA1. Based on the catalytic reduction of silver ion by AuNPs, a silver layer was formed on the surface of AuNPs. The quantitative assay of ·OH was carried out by differential pulse voltammetry (DPV) detection of the deposited silver. Under the optimization conditions, the developed DNA-based biosensor could detect ·OH quantitatively with wide linear range (0.2 ~ 200 μmol·L^-1) and low detection limit (50 nmol·L^-1), and exhibited satisfactory selectivity and reproducibility. This electrochemical biosensor could have potential application in the evaluation of antioxidant capacity.

Key words: hydroxyl radical, DNA damage, gold nanoparticle, silver-enhancement, electrochemical sensor

中图分类号:

O646

杨妍，喻鹏，张小华*，陈金华*. “金标银染”放大技术的羟基自由基灵敏检测[J]. 电化学（中英文）, 2015, 21(1): 22-28.

YANG Yan, YU Peng, ZHANG Xiao-hua*, CHEN Jin-hua*. Sensitive Detection of Hydroxyl Radical based on Silver-Enhanced Gold Nanoparticle Label[J]. Journal of Electrochemistry, 2015, 21(1): 22-28.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.140442

https://electrochem.xmu.edu.cn/CN/Y2015/V21/I1/22

参考文献

[1] Mello L D, Hernandez S, Marrazza G, et al. Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor[J]. Biosensors and Bioelectronics, 2006, 21(7): 1374-1382.
[2] Valko M, Izakovic M, Mazur M, et al. Role of oxygen radicals in DNA damage and cancer incidence[J]. Molecular and Cellular Biochemistry, 2004, 266(1/2): 37-56.
[3] King P, Anderson V, Edwards J, et al. A stable solid that generates hydroxyl radical upon dissolution in aqueous solutions: Reaction with proteins and nucleic acid[J]. Journal of the American Chemical Society, 1992, 114(13): 5430-5432.
[4] Cadet J, Delatour T, Douki T, et al. Hydroxyl radicals and DNA base damage[J]. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1999, 424(1): 9-21.
[5] Bandyopadhyay U, Das D, Banerjee R K. Reactive oxygen species: Oxidative damage and pathogenesis[J]. Current Science, 1999, 77(5): 658-666.
[6] Raha S, Robinson B H. Mitochondria, oxygen free radicals, disease and ageing[J]. Trends in Biochemical Sciences, 2000, 25(10): 502-508.
[7] Loeb L A, Wallace D C, Martin G M. The mitochondrial theory of aging and its relationship to reactive oxygen species damage and somatic mtDNA mutations[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(52): 18769-18770.
[8] Newton G L, Milligan J R. Fluorescence detection of hydroxyl radicals[J]. Radiation Physics and Chemistry, 2006, 75(4): 473-478.
[9] Pou S, Ramos C L, Gladwell T, et al. A kinetic approach to the selection of a sensitive spin trapping system for the detection of hydroxyl radical[J]. Analytical Biochemistry, 1994, 217(1): 76-83.
[10] Pritsos C A, Constantinides P P, Tritton T R, et al. Use of high-performance liquid chromatography to detect hydroxyl and superoxide radicals generated from mitomycin C[J]. Analytical Biochemistry, 1985, 150(2): 294-299.
[11] Yu J, Ge L, Huang J, et al. Microfluidic paper-based chemiluminescence biosensor for simultaneous determination of glucose and uric acid[J]. Lab on A Chip, 2011, 11(7): 1286-1291.
[12] Liu Y, Hu N. Electrochemical detection of natural DNA damage induced by ferritin/ascobic acid/H2O2 system and amplification of DNA damage by endonuclease Fpg[J]. Biosensors and Bioelectronics, 2009, 25(1): 185-190.
[13] Labuda J, Bu?ková M, Heilerova L, et al. Detection of antioxidative activity of plant extracts at the DNA-modified screen-printed electrode[J]. Sensors, 2002, 2(1): 1-10.
[14] Huang J, Li T, Chen Z, et al. Rapid electrochemical detection of DNA damage and repair with epigallocatechin gallate, chlorogenic acid and ascorbic acid[J]. Electrochemistry Communications, 2008, 10(8): 1198-1200.
[15] Zu Y, Liu H, Zhang Y, et al. Electrochemical detection of in situ DNA damage with layer-by-layer films containing DNA and glucose oxidase and protection effect of catalase layers against DNA damage[J]. Electrochimica Acta, 2009, 54(10): 2706-2712.
[16] Wu L, Yang Y, Zhang H, et al. Sensitive electrochemical detection of hydroxyl radical with biobarcode amplification[J]. Analytica Chimica Acta, 2012, 756: 1-6.
[17] Wang Y, Yin X, Shi M, et al. Probing chiral amino acids at sub-picomolar level based on bovine serum albumin enantioselective ?lms coupled with silver-enhanced gold nanoparticles[J]. Talanta, 2006, 69(5): 1240-1245.
[18] Chen Z, Peng Z, Luo Y, et al. Successively ampli?ed electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement[J]. Biosensors and Bioelectronics, 2007, 23(4): 485-491.
[19] Lin L, Liu Y, Tang L, et al. Electrochemical DNA sensor by the assembly of graphene and DNA-conjugated gold nanoparticles with silver enhancement strategy[J]. Analyst, 2011, 136(22): 4732-4737.
[20] Deng C, Chen J, Nie Z, et al. Impedimetric aptasensor with femtomolar sensitivity based on the enlargement of surface-charged gold nanoparticles[J]. Analytical Chemistry, 2008, 81(2): 739-745.
[21] Huang W T, Xie W Y, Shi Y, et al. A simple and facile strategy based on Fenton-induced DNA cleavage for fluorescent turn-on detection of hydroxyl radicals and Fe2+[J]. Journal of Materials Chemistry, 2011, 22(4): 1477-1481.
[22] Huang Y, Wang T H, Jiang J H, et al. Prostate specific antigen detection using microgapped electrode array immunosensor with enzymatic silver deposition[J]. Clinical chemistry, 2009, 55(5): 964-971.
[23] Huang C C, Chiu S H, Huang Y F, et al. Aptamer-functionalized gold nanoparticles for turn-on light switch detection of platelet-derived growth factor[J]. Analytical Chemistry, 2007, 79(13): 4798-4804.
[24] Jia S P, Liang M M, Guo L H. Photoelectrochemical detection of oxidative DNA damage induced by Fenton reaction with low concentration and DNA-associated Fe2+[J]. The Journal of Physical Chemisty B, 2008, 112(14): 4461-4464.
[25] Liang M M, Guo L H. Photoelectrochemical DNA sensor for the rapid detection of DNA damage induced by styrene oxide and the Fenton reaction[J]. Environmental Science & Technology, 2007, 41(2): 658-664.

“金标银染”放大技术的羟基自由基灵敏检测

Sensitive Detection of Hydroxyl Radical based on Silver-Enhanced Gold Nanoparticle Label

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

[1]	罗大娟, 刘冰倩, 覃蒙颜, 高荣, 苏丽霞, 苏永欢. 基于Au/rGO/FeOOH的新型电化学传感器一步检测亚硝酸盐[J]. 电化学（中英文）, 2022, 28(8): 2110191-.
[2]	余志远, 黄蕊, 刘杰, 李广, 宋前通, 孙世刚. PdCoIr四面体合金纳米催化剂的制备及其对乙醇氧化的电催化性能[J]. 电化学（中英文）, 2021, 27(1): 63-75.
[3]	邢逸飞, 李娜, 温晓芳, 韩宏彦, 崔敏, 张聪, 任聚杰, 籍雪平. 基于取代型多酸复合材料的多巴胺电化学检测[J]. 电化学（中英文）, 2020, 26(6): 890-899.
[4]	马武威, 常启刚, 史雄芳, 童延斌, 周立, 叶邦策, 鲁建江, 赵金虎. 基于纳米孔金与离子印迹聚合物结合的新型电化学传感器用于测定砷离子(III)[J]. 电化学（中英文）, 2020, 26(6): 900-910.
[5]	王来玉, 奚馨, 吴东清, 刘雄宇, 纪伟, 刘瑞丽. 有序介孔碳/石墨烯/镍泡沫的制备及其对多巴胺的高灵敏度和高选择性检测[J]. 电化学（中英文）, 2020, 26(3): 347-358.
[6]	彭劲骥, 郑红, 邹义松, 刘国坤, 袁东星. 基于电化学方法测定饮用水源水中的痕量铜离子[J]. 电化学（中英文）, 2019, 25(6): 699-707.
[7]	农永玲, 乔妮娜, 梁营. 基于AuNPs/PANI/TNTs纳米复合材料的电化学检测妥布霉素的适配体传感器[J]. 电化学（中英文）, 2019, 25(6): 720-730.
[8]	戴琬琳, 鲁志伟, 叶建山. 二次刻蚀聚酰亚胺负载Cu_xO纳米复合物薄膜电极用于葡萄糖的快速测定[J]. 电化学（中英文）, 2019, 25(2): 260-269.
[9]	董鹏飞，李娜，赵海燕，崔敏，张聪，任聚杰，藉雪平. Keggin 型磷钨酸盐修饰碳糊电极传感多巴胺的研究[J]. 电化学（中英文）, 2018, 24(5): 555-562.
[10]	陈家丽，张霞光，吴德印，田中群. 水助催化氢分子在金纳米粒子上解离的理论研究[J]. 电化学（中英文）, 2018, 24(3): 199-206.
[11]	王慧娟. 超薄Co₃O₄纳米片薄膜制备及其电化学传感器性能[J]. 电化学（中英文）, 2016, 22(6): 631-635.
[12]	王春燕*，刘晓秋，戚颖欣. 基于对羟基苯硼酸为前驱体的金纳米粒子修饰电极电化学检测过氧化氢[J]. 电化学（中英文）, 2016, 22(1): 88-93.
[13]	彭花萍, 余美玲, 刘馨, 刘盼, 陈伟, 刘爱林, 林新华*. 金纳米粒子/聚多巴胺/碳纳米管修饰玻碳电极对核黄素的测定[J]. 电化学（中英文）, 2016, 22(1): 43-48.
[14]	林丽清，赵成飞，蒋周倩，翁少煌，谢晓兰，林新华*. 基于DNA聚合酶I的新型电化学传感器及其胰腺癌K-ras基因点突变检测[J]. 电化学（中英文）, 2015, 21(1): 72-77.
[15]	黄春芳，姚桂红，邱建丁*. 表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用[J]. 电化学（中英文）, 2014, 20(6): 521-526.