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电化学(中英文) ›› 2007, Vol. 13 ›› Issue (2): 203-206.  doi: 10.61558/2993-074X.1808

• 研究论文 • 上一篇    下一篇

血红蛋白在月桂酸修饰玻碳电极的电催化行为

苗新蕊;张旭红;谢英;   

  1. 华南理工大学材料科学与工程学院,河北师范大学化学与材料科学学院,河北师范大学化学与材料科学学院 广东广州,510640,河北石家庄,050016,河北石家庄,050016
  • 收稿日期:2007-05-28 修回日期:2007-05-28 出版日期:2007-05-28 发布日期:2007-05-28

Voltammetric Behavior and Electrocatalytic Properties of Hemoglobin at Glassy Carbon Electrode Modified with Lauric Acid

MIAO Xin-rui1,ZHANG Xu-hong2,XIE Ying2   

  1. (1.College of Materials Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China,2.College of Chemistry and Material Science,Hebei Normal University,Shijiazhuang 050016,Hebei,China
  • Received:2007-05-28 Revised:2007-05-28 Published:2007-05-28 Online:2007-05-28

摘要: 研究血红蛋白在月桂酸修饰电极上的电化学行为,在0.02mol.L-1KH2PO4-Na2HPO4(pH=7)的缓冲液中,+0.6~-0.7V(vs.Ag/AgCl)电位范围内,Hb于该修饰电极产生不可逆还原电流峰.还原峰电流ip与v1/2呈线性关系,ip随溶液pH值和血红蛋白浓度的增加而增大,其浓度在1.00×10-8~5.00×10-9mol.L-1和1.92×10-6~2.06×10-7mol.L-1范围内分段呈线性变化关系.实验数据经进一步分析拟合,得到更精确的信息.该电极可作为检测血红蛋白的新型电化学生物传感器.

关键词: 血红蛋白, 循环伏安, 修饰电极, 月桂酸

Abstract: The electrochemical behavior of hemoglobin at glassy carbon(GC)electrode modified with Lauric Acid was studied.In the potential of +0.6~-0.7 V(vs.Ag/AgCl),an irreversible cathodic peak was observed at-0.3 V at the scan rate of 20 mV·s-1.In the medium of 0.02 mol·L-1 KH2PO4-Na2HPO4(pH 7.0),ip increased with the increasing concentration of Hb and the value of pH.The peak currents were proportional to the concentrations of hemoglobin in the range of 1.00×10-8~5.00×10-9 mol·L-1 and 1.92×10-6~2.06×10-7 mol·L-1.The data was analyzed in details by simulating in order to obtain more information and reaction mechanism of Hb on modified electrode.The electrode can be used as an approach for determining hemoglobin with high sensitivity.

Key words: hemoglobin, cyclic voltammetry, modified electrode, lauric acid

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