Fe(CN)63-/4-和细胞色素c电化学反应温度影响比较

doi:10.61558/2993-074X.2878

电化学（中英文） ›› 2012, Vol. 18 ›› Issue (1): 43-50. doi: 10.61558/2993-074X.2878

Fe(CN)63-/4-和细胞色素c电化学反应温度影响比较

汤儆*，杜琳, 肖孝建，张宁

福州大学化学化工学院, 教育部暨福建省食品安全和分析检测重点实验室, 福建福州, 350108

收稿日期:2011-09-21 修回日期:2011-10-17 出版日期:2012-02-28 发布日期:2011-11-01
通讯作者: 汤儆 E-mail:jingtang@fzu.edu.cn
基金资助:
国家自然科学基金项目（20873112，21073038）资助

Temperature Effects on the Electron-Transfer Reactions of Fe(CN)63-/4- and Cytochrome c

TANG Jing*, DU Lin, XIAO Xiao-Jian, ZHANG Ning

Key Laboratory of Analysis and Detection Technology for Food Safety, Ministry of Education, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou,350108, China

Received:2011-09-21 Revised:2011-10-17 Published:2012-02-28 Online:2011-11-01
Contact: TANG Jing E-mail:jingtang@fzu.edu.cn

摘要/Abstract

摘要： 应用整体升温的方法结合循环伏安和电位阶跃实验技术研究了温度对Fe(CN)63-/4-体系电化学反应的影响，计算不同温度下Fe(CN)63-和Fe(CN)64-的扩散系数D以及异相反应速率常数k0。实验发现，在Fe(CN)63-/4-体系中，Fe(CN)63-的扩散系数随着温度的升高而增大，而Fe(CN)64-的扩散系数则因受到Fe(CN)63-影响而出现相反的趋势。同时，异相反应速率常数也随温度升高而呈现下降趋势，推测这是由于电极表面生成了普鲁士蓝。由于细胞色素c的核心是血红素铁卟啉环，电子的得失都关联铁原子的氧化还原，与Fe(CN)63-/4-体系的电化学反应在一定程度上有其相似性，本文同时应用整体升温的方法研究了温度对细胞色素c电化学反应的影响，计算不同温度下细胞色素c的动力学参数，并与Fe(CN)63-/4-工作比较。

关键词: 温度效应, 细胞色素c, 扩散系数, 异相反应速率常数

Abstract: Cyclic voltammetric and chronoamperometric techniques were employed to study the temperature effects on the electrochemical reaction of Fe(CN)63-/4- by self-made thermostatic water bath electrochemical cell. The diffusion coefficient D and the formal heterogeneous electron-transfer rate constant k0 were calculated at different temperatures from 25 °C to 75 °C. In the Fe(CN)63-/4- system, the DO values of Fe(CN)63- were directly proportional to temperature, while DR of Fe(CN)64- inversely proportional to temperature. The values of k0 decreased with the increase of temperature due possibly to the electrode passivation resulted from the decomposition of Fe(CN)63-/4- to a Prussian blue like film. Since the core of Cytochrome c is the heme iron porphyrin, the redox of Cytochrome c reflects that of the iron atoms, which is, in certain degrees, similar to the electrochemical reactions of Fe(CN)63-/4- system. Accordingly, the same techniques were used to study the temperature effects on Cytochrome c. The kinetic parameters of Cytochrome c at different temperature were also calculated and compared with those of Fe(CN)63-/4- system.

Key words: Temperature effect, Cytochrome c, Diffusion coefficients, formal heterogeneous electron-transfer rate constant

中图分类号:

O657.1

汤儆, 杜琳, 肖孝建, 张宁. Fe(CN)63-/4-和细胞色素c电化学反应温度影响比较[J]. 电化学（中英文）, 2012, 18(1): 43-50.

TANG Jing, DU Lin, XIAO Xiao-Jian, ZHANG Ning. Temperature Effects on the Electron-Transfer Reactions of Fe(CN)63-/4- and Cytochrome c[J]. Journal of Electrochemistry, 2012, 18(1): 43-50.

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Fe(CN)63-/4-和细胞色素c电化学反应温度影响比较

Temperature Effects on the Electron-Transfer Reactions of Fe(CN)63-/4- and Cytochrome c

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