关键词: 微带阵列电极, 微型葡萄糖传感器, 葡萄糖氧化酶, Nafion, 二茂铁

Abstract: The microband array electrode(MAE) consisting of 11 Pt microband of 5 m in wide, 2000 m long and 85 m gap was fabricated with a thin film techology .The MAE was characterized by means of cyclic voltammetry in a solution of K3Fe(CN)6 and H2O2. The results showed higher current density on MAE than on conventional Pt eletrode due to the strong edge effect of the former. A glucose microsensor was constructed with the substrate electrode of microband array. Microband array electrode was modified by Nafion followed by the immobilization of ferrocene onto Nafion layer as the electron transfer mediator,finally a glucose microsensor was constructed by using bovine serum albumin and glutaraldehyde to immobilize glucose oxidase onto the surface of Nafion_Fc modified microband array electrode. The upper detection limit of the microsensor was 8.0 mmol/L and the response time was less than 10s.No significant variation was observed within two weeks use in detection.