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电化学(中英文) ›› 2008, Vol. 14 ›› Issue (4): 383-387.  doi: 10.61558/2993-074X.1926

• 研究论文 • 上一篇    下一篇

氯过氧化物酶在碳纳米管修饰电极上的直接电化学研究

张红霞;吴霞琴;后雯璟;谢文;陆中庆;王荣;李和兴;   

  1. 上海师范大学化学系;
  • 收稿日期:2008-11-28 修回日期:2008-11-28 出版日期:2008-11-28 发布日期:2008-11-28

Direct Electrochemistry of Chloroperoxidase at GC Electrode Modified with SWNTs

ZHANG Hong-xia,WU Xia-qin*,HOU Wen-jing,XIE Wen,LU Zhong-qing,WANG Rong,LI He-xing   

  1. (Department of Chemistry,Shanghai Normal University,Shanghai 200234,China
  • Received:2008-11-28 Revised:2008-11-28 Published:2008-11-28 Online:2008-11-28

摘要: 将氯过氧化物酶固定到单壁碳纳米管修饰玻碳电极,其循环伏安曲线出现一对准可逆氧化还原峰,说明碳纳米管能够很好地促进氯过氧化物酶在电极表面的直接电子传递.该过程与溶液pH值有关,可指认氯过氧化物酶在电极表面发生的是一电子一质子传递反应.该修饰电极制作简单,性能稳定,且对氧还原具有很好的电催化效应.

关键词: 氯过氧化物酶, 碳纳米管, 直接电化学, 修饰电极, 氧还原, 电催化

Abstract: The chloroperoxidase(CPO) was immobilized onto the surface of SWNTs modified electrodes.The electrochemical behaviors of modified electrode have been investigated by cyclic voltammetry.The appearance of a pair of well-defined redox peaks in the CV curve indicated that SWNTs can promote the electron transfer reaction of CPO.The results of cyclic voltammetry in PBS of different pH,from 3~9,showed that the reaction of CPO on the modified electrode was with equal numbers of electron and protein.This readily prepared SWNTs modified electrode showed excellent stability and good catalysis to O2.

Key words: chloroperoxidase, carbon nanotubes, direct electrochemistry, modified electrode, oxygen reduction, electrocatalysis

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