电化学(中英文) ›› 2016, Vol. 22 ›› Issue (3): 299-305. doi: 10.13208/j.electrochem.150914
廖 妮1*,卓 颖2,袁 若2
收稿日期:
2015-09-14
修回日期:
2015-11-10
出版日期:
2016-06-28
发布日期:
2015-11-18
通讯作者:
廖 妮
E-mail:liaoni123456789@163.com
作者简介:
廖 妮
基金资助:
国家自然科学基金项目(No. 21575116)资助
LIAO Ni1*, ZHUO Ying2, YUAN Ruo2
Received:
2015-09-14
Revised:
2015-11-10
Published:
2016-06-28
Online:
2015-11-18
Contact:
LIAO Ni
E-mail:liaoni123456789@163.com
About author:
LIAO Ni
摘要:
采用一锅合成法制备了新型的具有大比表面积的花状铂纳米颗粒(PtNFs),并构建了一个高灵敏电致化学发光(ECL)免疫传感器用于检测载脂蛋白A1(Apo-A1). 该PtNFs用于吸附二抗(anti-Apo-A1),并用葡糖糖氧化酶(GOD)封闭其表面的非特异性位点,最终制备了PtNFs@anti-Apo-A1@GOD信号探针. 当Apo-A1存在时,通过夹心免疫反应将制备的信号探针捕获于电极表面,并将所制得的电极置于含有葡萄糖的过硫酸根底液中检测. GOD催化葡萄糖产生H2O2,H2O2在PtNFs的催化下分解并在电极表面原位产生O2,所产生的O2能够催化过硫酸根-氧气体系的电致化学发光反应,放大发光信号,提高检测灵敏度. 该传感器在0.1 ng•mL-1 ~ 100 ng•mL-1范围内对Apo-A1有良好的线性响应,检测下限达到0.03 ng•mL-1,有望应用于临床分析诊断.
中图分类号:
廖 妮,卓 颖,袁 若. 基于花状铂纳米颗粒构建的电致化学发光免疫传感器用于检测载脂蛋白A1[J]. 电化学(中英文), 2016, 22(3): 299-305.
LIAO Ni, ZHUO Ying, YUAN Ruo. Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1[J]. Journal of Electrochemistry, 2016, 22(3): 299-305.
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