关键词: 花状铂纳米颗粒, 电致化学发光免疫传感器, 载脂蛋白A1

Abstract:

In this paper, a novel electrochemiluminescence (ECL) immunosensor for the detection of apolipoprotein A1 was constructed based on flower-like platinum nanoparticles (PtNFs) via a one-pot chemical synthesis method. The PtNFs was used to immobilize the secondary antibody and enzyme (GOD). Then the prepared bioconjugates were introduced onto the electrode via sandwich immunoreactions. Accordingly, the ECL luminophore peroxydisulfate (S₂O₈^2- ) was presented in the working buffer solution containing an appropriate amount of glucose. Through the ECL responses of S₂O₈^2- and O₂, a dramatically amplified ECL signal was obtained for the reason that hydrogen peroxide (H₂O₂) produced by GOD to glucose was subsequently catalyzed by PtNFs to in situ generate O₂. The present immunosensor showed a wide linear range of 0.1  ng•mL^-1 to 100  ng•mL^-1, with a low detection limit of 0.03  ng•mL^-1 for detecting Apo-A1. This new signal amplification strategy for preparation of ECL immunosensor could be easily realized and has potential application in ultrasensitive bioassays.

Key words: flower-like structures platinum nanoparticles, electrochemiluminescence immunosensor, apolipoprotein A1