关键词: 废旧金属网, 微生物电解池, 产氢性能, 16S rDNA扩增测序

Abstract: In order to find high quality and low price hydrogen catalyst, a scrap metal net was used as single-chamber microbial electrolytic cell (MEC) cathode, and its hydrogen production performance was investigated at different applied voltages. Meanwhile, the microbial community structures of original aerobic activated sludge, MFC and MEC anode microbes were analyzed by 16S rDNA amplicon sequencing technology. As the applied voltage increased, the maximum current density was increased and the running time was shortened. At an applied voltage of 0.7 V, the hydrogen production and electrical energy efficiency obtained with scrap metal mesh cathode MEC were, respectively, 0.330±0.012 m³H₂·m^-3·d^-1 and 177.0±5.6%, which were much higher than those at 0.5 V, but similar to those at 0.9V. The performance of waste metal mesh cathode MEC was comparable to that of Pt/C cathode MEC, and had good durability. The results of 16S rDNA amplicon sequencing showed that the culture environment had a great influence on the enrichment and elimination of microorganisms. Geobacter, the dominant bacterium of MEC anode, was extremely enriched in the applied electric field environment with a relative abundance of more than 79.4%.

Key words: scrap metal net, microbial electrolytic cell, hydrogen-producing performance, 16S rDNA amplicon sequencing