关键词: 分子包合作用, 超分子电化学, 聚环糊精, 非生理性电子受体, 酶电极

Abstract: The inclusion complexation of α-cyclodextin prepolymer host with benzoquinone guest was studied by voltametry. The spectroscopic measurements show that the polycondensation of CD with glutaric dialdehyde forms the polymer (PCD) with pending carbonyl, the latter brings about covalent immobilisation of glucose oxidase (GOD) to polymer chains. Owing to the inclusion complexation of CD, the benzoquinone(BQ) as mediator of GOD can be collected in the enzymecontaining PCD membrane, resulting in high concentration of BQ in the membrane while decreasing its amount in solution. TTF was tested to prepare the immobilised enzyme together with mediator in PCD. The enzyme loading in PCD membrane and the membrane thickness can be controlled easily, which will facilate to fundmental reshearch of enzyme electrodes.