石墨烯修饰玻碳电极用于急性早幼粒细胞白血病PML/RARa融合基因的检测

doi:10.61558/2993-074X.2089

电化学（中英文） ›› 2011, Vol. 17 ›› Issue (2): 195-198. doi: 10.61558/2993-074X.2089

石墨烯修饰玻碳电极用于急性早幼粒细胞白血病PML/RARa融合基因的检测

连志贤1，钟光贤1，王昆1, 3，刘爱林1, 2*，林新华1*，陈元仲2

1.福建医科大学药学院,药物分析系, 福建福州 350004;
2. 福建医科大学附属协和医院福建省血研所，福建福州 350001；
3. 厦门大学附属第一医院药剂科，福建厦门 361003

收稿日期:2011-01-14 修回日期:2011-02-25 出版日期:2011-05-28 发布日期:2011-05-06
通讯作者: 刘爱林; 林新华 E-mail:ailinliu@mail.fjmu.edu.cn; xhl1963@sina.com
基金资助:
国家863计划资助项目(2008AA02Z433)；国家自然科学基金资助项目(20805006，20975021)；福建省高校产学研科技重点项目(2010Y4003)；福建省自然科学基金资助项目(2010J06011)；福建省血液病学重点实验室项目(2009J1004)；福建医科大学校重大项目(09ZD013)；福建省高校杰出青年科研人才计划(JA10126)

Detection of Acute Promyelocytic Leukemia PML/RARα Fusion Gene Using Graphene Modified Glassy Carbon Electrode

LIAN Zhi-xian1, ZHONG Guang-xian1, WANG Kun1,3, LIU Ai-lin1,2*, LIN Xin-hua1*, CHEN Yuan-zhong2

1. Department of Pharmaceutical Analysis of the Fujian Meidical University, Fuzhou 350004,China;
2. Fujian Institute of Hematology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China;
3.Department of Pharmacy, the First Hospital of Xiamen Affiliated to the Xiamen University, Xiamen 361003, Fujian, China

Received:2011-01-14 Revised:2011-02-25 Published:2011-05-28 Online:2011-05-06
Contact: LIU Ai-lin, LIN Xin-hua E-mail:ailinliu@mail.fjmu.edu.cn; xhl1963@sina.com

摘要/Abstract

摘要： 应利用电化学还原法将固定在玻碳电极表面的氧化石墨还原为石墨烯，然后再利用偶联活化剂将氨基修饰的急性早幼粒细胞白血病（APL）PML／RARα融合基因序列探针固定到石墨烯修饰电极表面，以亚甲基蓝（MB）为电化学杂交指示剂，并由差分脉冲伏安法检测人工合成APL的PML／RARα融合基因.结果表明，石墨烯对MB的检测信号起到了很好的增敏作用，杂交前后MB还原峰电流差值与靶标链DNA浓度在5×10-10~2.5×10-9 mol/L范围内呈线性关系，检出限为8×10-11 mol/L.该方法简单、特异性好，有望用于实际样品的检测.

关键词: 石墨烯, 电化学DNA传感器, PML/RAR&alpha, 融合基因

Abstract: A graphene modified glassy carbon electrode (GCE) was prepared by electrochemical reduction of graphite oxide fixed on the surface of GCE. Then, the amino-group modified probe of acute promyelocytic leukemia (APL) PML/RARα fusion gene fragment was immobilized onto surface of the graphene modified GCE by coupling activating agent. With methylene blue (MB) as a novel electrochemical indicator, artificial APL PML/RARα fusion gene fragment was measured by differential pulse voltammetry (DPV). The result indicated graphene nanomaterial showed good sensitization on the signal of MB. The relationship between the reduction peak current of MB and the concentration of complementary strand was linear in the range of 5×10-10~2.5×10-9 mol/L. The detection limit had been estimated as 8×10-11 mol/L. The method is simple, good specificity and high sensitivity. This approach is promising to the detection of APL in the real sample for medical diagnostics.

Key words: Graphene, electrochemical DNA biosensor, PML/RAR&alpha, fusion gene

中图分类号:

O657. 1

连志贤, 钟光贤, 王昆, 刘爱林, 林新华, 陈元仲. 石墨烯修饰玻碳电极用于急性早幼粒细胞白血病PML/RARa融合基因的检测[J]. 电化学（中英文）, 2011, 17(2): 195-198.

LIAN Zhi-xian, ZHONG Guang-xian, WANG Kun, LIU Ai-lin, LIN Xin-hua, CHEN Yuan-zhong. Detection of Acute Promyelocytic Leukemia PML/RARα Fusion Gene Using Graphene Modified Glassy Carbon Electrode[J]. Journal of Electrochemistry, 2011, 17(2): 195-198.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://electrochem.xmu.edu.cn/CN/10.61558/2993-074X.2089

https://electrochem.xmu.edu.cn/CN/Y2011/V17/I2/195

参考文献

[1] GeimA K，Novoselov K S．The rise of graphene[J]. Nature Mater，2007, 6(3): 1 183 - 191.

[2] Shan C S, Yang H F, Han D X, et al. Water-Soluble Graphene Covalently Functionalized by Biocompatible Poly-L-lysine [J]. Langmuir, 2009, 25(20) :12030-12033.

[3] Fu C L, Yang W S, Chen X, et al．Direct electrochemistry of glucose oxidase on a graphite nanosheet-Nafion composite film modified electrode[J]. Electrochem Commun, 2009, 11(5)：997-1000.

[4] Zhang J Y(张建瑜)，Tang J M (唐加明). Clinic study about 21 cases of acute promyelocytic leukemia [J]. International Medicine & Health Guidance News (国际医药卫生导报), 2007, 13(12): 28-31.

[5] Yoo S J, Seo E J, Lee J H, et al. A complex, four-way variant t(15; 17) in acute promyelocytic leukemia[J]. Cancer Genet Cytogenet, 2006, 167 (2): 168-171.

[6] Han J Y, Kim K E, Kim K H, et al. Identification of PML-RARA rearrangementbyRT-PCR and sequencing in an acute promyelocytic leukemiawithout t(15; 17)on G-banding and FISH[ J]. Leukemia Research, 2007, 31(2): 239-243.

[7] Wei N, Chen J H, Zhang J, et al. An electrochemical biosensor for detection of PML/RARA fusion gene using capture probe covalently immobilized onto poly-calcon carboxylic acid modified glassy carbon electrode [J]. Talanta, 2009, 78 (4-5): 1227-1234.

[8] Guo H L, Wang X F, Qian Q Y, et al. A Green approach to the synthesis of graphene nanosheets[J]. ACS Nano, 2009, 3 (9) : 2653–2659.

[9] Hummers W S, Offeman R E. Preparation of graphite oxide [J]. J Am Chem Soc, 1958, 80(6):1339-1339．

[10] Nethravathi C, Viswanath B, Shivakumara C, et al. The production of semectite clay/graphene composites through delamination and co-stacking[J]. Carbon, 2008, 46(13): 1773-1781.

[11] Erdem, A, Kerman K, Meric B, et al. Methylene blue as a novel electrochemical hybridization indicator[J]. Electroanalysis, 2001, 13(3):219-223.

[12] Lin X H, Wu P, Chen W, et al. Electrochemical DNA biosensor for the detection of short DNA species of chronic myelogenous leukemia by using methylene blue [J]. Talanta, 2007, 72 (3): 468-471.

[1]	崔苗苗, 韩联欢, 曾兰平, 郭佳瑶, 宋维英, 刘川, 吴元菲, 罗世翊, 刘云华, 詹东平. 单层石墨烯微米尺度图案化和功能化：调控电子传输特性[J]. 电化学（中英文）, 2024, 30(3): 2305251-.
[2]	刘双娟, 王海静, 郭靖, 王鹏程, 周昊, 孟才, 郭汉杰. 电沉积法制备石墨烯纸-金属复合材料的初步研究[J]. 电化学（中英文）, 2021, 27(4): 396-404.
[3]	张运丰, 董佳明, 谭畅, 霍士康, 王佳颖, 何阳, 王雅莹. Li-SGO掺杂半互穿网络型多孔单离子传导聚合物复合电解质的制备[J]. 电化学（中英文）, 2021, 27(1): 108-117.
[4]	邢逸飞, 李娜, 温晓芳, 韩宏彦, 崔敏, 张聪, 任聚杰, 籍雪平. 基于取代型多酸复合材料的多巴胺电化学检测[J]. 电化学（中英文）, 2020, 26(6): 890-899.
[5]	陈品松, 胡一涛, 张信义, 沈培康. 立体构造石墨烯材料对铅酸蓄电池负极性能影响的研究[J]. 电化学（中英文）, 2020, 26(6): 834-843.
[6]	张泽阳, 孙岚, 林昌健. RGO-TiO₂纳米管阵列的制备及其光电性能[J]. 电化学（中英文）, 2020, 26(6): 844-849.
[7]	孟全华, 邓雯雯, 李长明. 类石墨烯类活性炭材料的简易合成及其在锂硫电池中的应用研究[J]. 电化学（中英文）, 2020, 26(5): 740-749.
[8]	王来玉, 奚馨, 吴东清, 刘雄宇, 纪伟, 刘瑞丽. 有序介孔碳/石墨烯/镍泡沫的制备及其对多巴胺的高灵敏度和高选择性检测[J]. 电化学（中英文）, 2020, 26(3): 347-358.
[9]	姚硕, 黄太仲, RizwanHaider, 房恒义, 于洁玫, 姜占坤, 梁栋, 孙玥, 原鲜霞. NiO@rGO负载钯、银纳米粒子用作氧还原催化剂[J]. 电化学（中英文）, 2020, 26(2): 270-280.
[10]	张波, 刘佳, 刘晓晨, 李德军. 硫在不同碳载体材料中的电化学性能研究[J]. 电化学（中英文）, 2019, 25(6): 749-756.
[11]	杨娟, 郎俊伟, 张鹏, 刘宝. 高温驱动氧化锰刻蚀制备纳米氧化锰-多孔石墨烯及其锂空气电池性能研究[J]. 电化学（中英文）, 2019, 25(5): 621-630.
[12]	李二岭, 杨发, 阮明波, 宋平, 徐维林. Fe-N共掺杂纳米碳材料的形貌对电化学还原反应的影响[J]. 电化学（中英文）, 2019, 25(4): 486-496.
[13]	酒琳娜, 程永强. 二氧化钛纳米颗粒/还原氧化石墨烯修饰玻碳电极在对硝基苯酚检测中的应用[J]. 电化学（中英文）, 2019, 25(4): 504-510.
[14]	颜冲, 寇华日, 颜波, 刘晓静, 李德军, 李喜飞. Ni/Mn₃O₄/NiMn₂O₄@RGO空心微球负极的制备及其储钠性能[J]. 电化学（中英文）, 2019, 25(1): 112-121.
[15]	夏永康, 顾明远, 杨红官, 于馨智, 鲁兵安. CVD 法制备三维石墨烯的电化学储能性能[J]. 电化学（中英文）, 2019, 25(1): 89-103.

石墨烯修饰玻碳电极用于急性早幼粒细胞白血病PML/RARa融合基因的检测

Detection of Acute Promyelocytic Leukemia PML/RARα Fusion Gene Using Graphene Modified Glassy Carbon Electrode

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics