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电化学(中英文) ›› 2002, Vol. 8 ›› Issue (3): 333-336. 

• 研究论文 • 上一篇    下一篇

硝酸还原酶在巯基自组装膜上的电化学性质

宋雅茹,吴红,邵会波   

  1. 北京师范大学化学系,北京师范大学化学系,首都师范大学化学系 北京100875 ,北京100875 ,北京100037
  • 收稿日期:2002-08-28 修回日期:2002-08-28 出版日期:2002-08-28 发布日期:2002-08-28

Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs

SONG Ya_ru 1,WU Hong 1,SHAO Hui_bo 2*   

  1. (1.Department of Chemistry,Beijing Normal University,Beijing 100875,China; 2.Department of Chemistry,Capital Normal University,Beijing 100037,China
  • Received:2002-08-28 Revised:2002-08-28 Published:2002-08-28 Online:2002-08-28

摘要: 应用自组装技术将硝酸还原酶 (NR)构筑在硫醇自组装单分子膜上 ,研究了Au/半胱胺 /NR和Au/半胱胺 /NR/卵磷脂两种酶电极在磷酸缓冲液中的直接电化学行为

关键词: 硝酸还原酶, 卵磷脂, 半脘胺自组装膜

Abstract: Nitrate reductase (NR) and phospholipids were immobilized on gold electrodes with 2_aminoethanethio(AET) SAMs. After Au/AET/NR and Au/AET/NR/lipid were soaked in pH 7 phosphate buffers for days,a pair of pre_reversible peak appeared at around 0.202 V and 0.149 V,respsctively, irregularly grew with soaking time in blank buffer.The cathodic peak current(i pcl )of Au/AET/NR system had linear relationship with scan rate between 0.02 and 0.5 V.s -1 ,while i pcl was proportional to V 1/2 in Au/AET/NR/lipid system.The direct electron transfer of enzyme electrodes was strongly affected by pH.Compared with Au/AET,NR exhibited a higher electrochemical activity in Au/AET/lipid system.

Key words: Nitrate reductase, Phospholipid, 2_aminoethanethiol SAMs

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