关键词: 正弦波电磁场, 电生孔, 放线菌素-C, 光动力学, 协同效应, 癌细胞U-937, K-562

Abstract: Weak pulsating sinusoidal electromagnetic fields (PEMF) have been applied to change membrane permeation and induction of apoptosis (necrosis). In the case of suspension of human cancer cells U-937 and K-562 inside of Helmholtz-coils the amplitude of PEMF B=10 and at 39mT (at 50 Hz) was combined gradually with the cytotoxic agent actinomycin-C on one hand with its novel photodynamic activity on the other. Depending on temperature, pH-value and treatment time the necrosis of these cells was determined by means of trypan blue staining as well as the induced apoptosis by FACScan technique. Synergisms were discovered yielding high rates of cell death for three combinations:I.PEMF + hyperthermia or (and) hyperacidityII.PEMF + cytostatic drugs in the dark III.PEMF + cytostatic drugs irradiated by visible light (photodynamic effect)The selection of cytostatic drugs as actinomycin-C and some anthracyclines was to prove their photodynamic activity, which is suitable to enhance their cytotoxic effects.These results of inductive coupling (PEMF) were compared with effects of A.C. currents and electroporation by single D.C. pulses between solid electrodes. Contrary to these both methods the PEMF application has a noninvasive influence on the viability of cancer cells suitable therefore to support tumor therapy by this adjuvant bioelectrochemical method.