基于铁氰化钾电子媒介体及醛基吡啶盐的电化学免疫传感器

doi:10.13208/j.electrochem.170406

电化学（中英文） ›› 2018, Vol. 24 ›› Issue (2): 154-159. doi: 10.13208/j.electrochem.170406

基于铁氰化钾电子媒介体及醛基吡啶盐的电化学免疫传感器

胡霞，申有名，沈广宇*

湖南文理学院化学与材料工程学院，湖南常德，415000

收稿日期:2017-04-06 修回日期:2017-06-16 出版日期:2018-04-28 发布日期:2017-06-16
通讯作者: 沈广宇 E-mail:sgyrab@163.com
基金资助:
湖南省自然科学基金项目（No. 2016JJ6105）资助

A Label-free Immunosensor Based on K₃[Fe(CN)₆] and Aldehyde-Functionalized Pyridinium

HU Xia, SHEN You-ming, SHEN Guang-yu*

College of Chemistry and Material Engineering, Hunan University of Arts and Science, Changde 415000, Hunan，China

Received:2017-04-06 Revised:2017-06-16 Published:2018-04-28 Online:2017-06-16
Contact: SHEN Guang-yu E-mail:sgyrab@163.com

摘要/Abstract

摘要： 采用聚二烯丙基二甲基氯化铵(PDDA)将铁氰化钾电子媒介体固定在电极表面，构建免标记的电化学免疫传感器. 醛基吡啶盐不仅作为基底物质直接固定抗体，还可以很好地增强电极表面的导电性能. 将构建的传感器用于肿瘤标志物甲胎蛋白的检测. 其线性范围为0.01-20 ng·mL^-1，检测下限为0.004 ng·mL^-1（3 S/N）. 此传感器的构建简单方便、无标记、特异性好，为甲胎蛋白及其他肿瘤标志物提供了新的检测方法.

关键词: 电化学免疫传感器, K₃[Fe(CN)₆], 醛基吡啶盐, 甲胎蛋白

Abstract: A novel aldehyde-functionalized pyridinium was successfully synthesized. Using K₃[Fe(CN)₆] as a redox species modified on the surface of the electrode through Poly(diallyldimethylammonium chloride), we developed a label-free immunosensor based on aldehyde-functionalized pyridinium that was applied to simplify antibody immobilization on the electrode surface, in which no additional chemical cross-linking was required. In addition, the introduction of K₃[Fe(CN)₆] on the surface of electrode avoided the use of other redox species in the electrolyte solution. Under the optimal experimental conditions, differential pulse voltammetry (DPV) signals corresponding to different α-Fetoprotein (AFP) concentrations were measured. After antigens with different concentrations reacted with antibodies immobilized on the electrode surface, the peak current of DPV gradually decreased with increasing concentrations of AFP. The calibration curve showed a good linear relation in the range from 0.01 to 20 ng·mL^-1 with the linear regression equation of y = -3.23x + 22.42 (R²=0.9943). The detection limit is 0.004 ng·mL^-1 based on a signal-to-noise ratio of 3. The constructed immunosensor is simple, label-free and specific, thus, provides a good diagnostic tool for detection of α-Fetoprotein and other cancer markers.

Key words: electrochemical immunosensor, K₃[Fe(CN)₆], aldehyde-functionalized pyridinium, α-Fetoprotein

中图分类号:

O646
O657.1

胡霞，申有名，沈广宇. 基于铁氰化钾电子媒介体及醛基吡啶盐的电化学免疫传感器[J]. 电化学（中英文）, 2018, 24(2): 154-159.

HU Xia, SHEN You-ming, SHEN Guang-yu. A Label-free Immunosensor Based on K₃[Fe(CN)₆] and Aldehyde-Functionalized Pyridinium[J]. Journal of Electrochemistry, 2018, 24(2): 154-159.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.13208/j.electrochem.170406

https://electrochem.xmu.edu.cn/CN/Y2018/V24/I2/154

参考文献

[1] Lai WQ, Tang D P, Que X H, et al. Enzyme-catalyzed silver deposition on irregular-shaped gold nanoparticles for electrochemical immunoassay of alpha-fetoprotein[J]. Analytica Chimica Acta, 2012, 755: 62-68.

[2] Yin Z Z, Liu Y, Jiang L P, et al. Electrochemical immunosensor of tumor necrosis factor α based on alkaline phosphatase functionalized nanospheres[J]. Biosensors and Bioelectronics, 2011, 26(5): 1890-1894.

[3]Li R, Wu K B, Liu C X, et al. 4-Amino-1-(3-mercapto-propyl)-pyridine hexa?uorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement[J]. Analytical Chemistry, 2014, 86(11): 5300-5307.

[4] Lin X(林晓), Weng S H(翁少煌)袁Zhou J Z(周剑章)，et al. Electrochemical Immunosensor based on polycalconcarboxylic acid modified electrode for the determination of alpha-fetoprotein[J]. Journal of Electrochemistry(电化学), 2012,18(4):371-376.
[5] Zhang J, He J L, Xu W L, et al. A novel immunosensor for detection of μ-galactoside β-2, 6-sialyltransferase in serum based on gold nanoparticles loaded on Prussian blue-based hybrid nanocomposite film[J]. Electrochimica Acta, 2015, 156: 45-52.
[6] Lin J H, Wei Z J, Chu P F. A label-free immunosensor by controlled fabrication of monoclonal antibodies and gold nanoparticles inside the mesopores[J]. Analytical Biochemistry, 2012, 421(1): 97-102.

[7] Qiu J D, Liang R P, Wang R, et al. A label-free amperometric immunosensor based on biocompatible conductive redox chitosan-ferrocene/gold nanoparticles matrix[J]. Biosensenors and Bioelectronics, 2009, 25(4): 852-857.

[8] Feng T, Qiao X, Wang H, et al. A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe₃O₄@SiO₂ as labels[J]. Biosensors and Bioelectronics 2016, 79(4): 48-54.

[9] Han J M, Ma J, Ma Z F. One-step synthesis of graphene oxide–thionine–Au nanocomposites and its application for electrochemical immunosensing[J]. Biosensors and Bioelectronics, 2013, 47(10): 243-247.

[10] Shi W T, Ma Z F. A novel label-free amperometric immunosensor for carcinoembryonic antigen based on redox membrane[J], Biosensors and Bioelectronics. 2011, 26(6): 3068-3071.

[11] Weng S H, Chen M, Zhao C F, et al. Label-free electrochemical immunosensor based on K₃[Fe(CN)₆] as signal for facile and sensitive determination of tumor necrosis factor-alpha[J]. Sensors and Actuators B, 2013, 184: 1-7.

[12] Muthirulan P, Velmurugan R. Direct electrochemistry and electrocatalysis of reduced glutathione on CNFs–PDDA/PB nanocomposite film modified ITO electrode for biosensors[J]. Colloids and Surfaces B, 2011, 83(2): 347-354.

[13] Jiao S F, Jin J, Wang L. One-pot preparation of Au-RGO/PDDA nanocomposites and their application for nitrite sensing[J]. Sensors and Actuators B, 2015, 208(2): 36-42.

[14] Amatatongchai M, Sroysee W, Chairam S, et al. Simple flow injection for determination of sulfite by amperometric detection using glassy carbon electrode modified with carbon nanotubes–PDDA–gold nanoparticles[J]. Talanta, 2015, 133: 134-141.

[15] Plater M J, Barnes P, McDonald L K, et al. Hidden signatures: new reagents for developing latent fingerprints[J]. Organic and Biomolecular Chemistry, 2009, 7(8): 1633-1641.

[16] Wang X, Zhang Q Y, Li Z J, et al. Development of high-performance magnetic chemiluminescence enzyme immunoassay for α-fetoprotein (AFP) in human serum[J]. Clinica Chimica Acta, 2008, 393(2): 90-94.

[17] Kavosi B, Salimi A, Hallaj R, et al. A highly sensitive prostate-specific antigen immunosensor based on gold nanoparticles/PAMAM dendrimer loaded on MWCNTS/chitosan/ionic liquid nanocomposite[J]. Biosensors and Bioelectronics, 2014, 52: 20-28.

[18] Liu D, Wu F, Zhou C, et al. Multiplexed immunoassay biosensor for the detection of serum biomarkers-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots[J]. Sensors and Actuators B, 2013, 186: 235-243.

[19] Shen G, Hu X, Zhang S. A signal-enhanced electrochemical immunosensor based on dendrimer functionalized-graphene as a label for the detection of μ-1-fetoprotein[J]. Journal of Electroanalytical Chemistry, 2014, 717: 172-176.

[20] Lin J, Wei Z, Zhang H, et al. Sensitive immunosensor for the label-free determination of tumor marker based on carbon nanotubes/mesoporous silica and graphene modified electrode[J]. Biosensors and Bioelectronics, 2013, 41: 342-347.

基于铁氰化钾电子媒介体及醛基吡啶盐的电化学免疫传感器

A Label-free Immunosensor Based on K₃[Fe(CN)₆] and Aldehyde-Functionalized Pyridinium

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