基于新型聚钙羧酸修饰电极的甲胎蛋白电化学免疫传感器研究

doi:10.61558/2993-074X.2933

电化学（中英文） ›› 2012, Vol. 18 ›› Issue (4): 371-376. doi: 10.61558/2993-074X.2933

基于新型聚钙羧酸修饰电极的甲胎蛋白电化学免疫传感器研究

林晓¹, 翁少煌¹, 周剑章², 刘爱林¹, 林新华^1*, 游勇基^1*

1. 福建医科大学药学院药物分析学系，福建福州 350004； 2. 厦门大学固体表面物理化学国家重点实验室，化学化工学院化学系，福建厦门 361005

收稿日期:2012-01-10 修回日期:2012-02-13 发布日期:2012-02-20 出版日期:2012-08-28
通讯作者: 林新华, 游勇基 E-mail:xhl1963@sina.com, youyj@sina.com
基金资助:
福建省高校产学研科技重点项目(No. 2010Y4003)和福建省自然科学基金项目(No. 2011J05023)资助

Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein

LIN Xiao¹, WENG Shao-Huang¹, ZHOU Jian-Zhang², LIU Ai-Lin¹, LIN Xin-Hua^1*, YOU Yong-Ji^1*

1. Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China; 2. State Key Laboratory of Physical Chemistry of the Solid Surface and Department of Chemistry, College of Chemistry and Chemical Engeering, Xiamen University, Xiamen 361005, Fujian, China

Received:2012-01-10 Revised:2012-02-13 Online:2012-02-20 Published:2012-08-28
Contact: LIN Xin-Hua, YOU Yong-Ji E-mail:xhl1963@sina.com, youyj@sina.com

摘要/Abstract

摘要： 通过电聚合制得新型聚钙羧酸修饰电极并用于构建检测甲胎蛋白（AFP）的高灵敏电化学免疫传感器. 采用扫描电镜（SEM）、电化学交流阻抗（EIS）观察、表征修饰电极和AFP单克隆抗体（Ab1）固定前后的差异. 固定Ab1的电极与一定浓度的AFP、辣根过氧化物酶联AFP单克隆抗体（HRP-Ab2）反应，形成夹心型免疫复合物. 辣根过氧化物酶（HRP）催化3,3',5,5'-四甲基联苯胺（TMB）底物产生电流信号，实现AFP浓度的测定. 本检测方法灵敏度高，重现性好.

关键词: 聚钙羧酸修饰电极, 甲胎蛋白, 免疫传感器

Abstract: A highly sensitive electrochemical immunosensor based on novel electropolymerized polycalconcarboxylic acid modified electrode (poly-CCA/GC) has been fabricated for the detection of alpha-fetoprotein (AFP). The differences of the poly-CCA/GC electrodes before and after the immobilization of AFP antibody were characterized via scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The immobilization of antibody/antigen/antibody-HRP sandwich was formed on the poly-CCA modified electrode for the determination of special AFP concentrations. The electrochemical signals generated from the HRP which catalyzed the TMB substrate were measured. Experiment results indicated that the developed immunosensor showed a good sensitivity and precision.

Key words: polycalconcarboxylic acid modified electrode, alpha-fetoprotein, immunosensor

中图分类号:

O646

林晓, 翁少煌, 周剑章, 刘爱林, 林新华, 游勇基. 基于新型聚钙羧酸修饰电极的甲胎蛋白电化学免疫传感器研究[J]. 电化学（中英文）, 2012, 18(4): 371-376.

LIN Xiao, WENG Shao-Huang, ZHOU Jian-Zhang, LIU Ai-Lin, LIN Xin-Hua, YOU Yong-Ji. Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein[J]. Journal of Electrochemistry, 2012, 18(4): 371-376.

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https://electrochem.xmu.edu.cn/CN/Y2012/V18/I4/371

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基于新型聚钙羧酸修饰电极的甲胎蛋白电化学免疫传感器研究

Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein

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