银盘电极盐酸柔红霉素电化学行为及其与BSA的相互作用

doi:10.61558/2993-074X.2004

电化学（中英文） ›› 2009, Vol. 15 ›› Issue (3): 326-330. doi: 10.61558/2993-074X.2004

银盘电极盐酸柔红霉素电化学行为及其与BSA的相互作用

程红芬;张勇;刘杰;刘艳梅;

山西大学化学化工学院;

收稿日期:2009-08-28 修回日期:2009-08-28 出版日期:2009-08-28 发布日期:2009-08-28

The Electrochemical Behavior of Daunorubicin Hydrochloride on Galactic Disk Electrode and its Reaction with BSA

CHENG Hong-fen,ZHang Yong,LIU Jie,LIU Yan-mei

(School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China

Received:2009-08-28 Revised:2009-08-28 Published:2009-08-28 Online:2009-08-28

摘要/Abstract

摘要： 应用线性扫描伏安法和循环伏安法研究盐酸柔红霉素在银盘电极上的电化学行为及其与牛血清白蛋白(BSA)的相互作用.结果表明,在pH6.50的Britton-Robinson缓冲溶液中,盐酸柔红霉素有一灵敏的还原峰,峰电位Ep-0.64V(vs.SCE),缓冲液加入BSA后盐酸柔红霉素的还原峰峰电流下降,据此建立了BSA的电化学测定方法.在优化条件下,峰电流与BSA浓度于1.0×10-8～1.0×10-4mol·L-1(r=0.9965)范围内呈线性关系,检出限为5.0×10-9mol·L-1.同时还测定了盐酸柔红霉素与BSA的结合比和结合常数.

关键词: 盐酸柔红霉素, 银盘电极, 电化学行为, 牛血清白蛋白(BSA)

Abstract: This article used the linear sweep voltammetry and the circulation voltammetry to study the electrochemical behavior of daunorubicin hydrochloride on galactic disk electrode and its electrode reaction with BSA.Also it studied the interaction of daunorubicin hydrochloride with BSA.The results showed that daunorubicin hydrochloride has a sensitive reduction peak at -0.64 V(vs. SCE) in pH 6.50 Britton-Robinson buffer.The valus of reductions peak current reduced by the addition of BSA to the above mentioned solution.A new electrochemical determination method of bovine serum albumin was developed based on the interaction of daunorubicin hydrochloride with BSA.Furthermore,under the optimized condition,the decrease of the reduction peak current of daunorubicin hydrochloride is proportional to the concentration of BSA in the range of 5.0×10-8 mol·L-1~1.0×10-4 mol·L-1(r=0.9965).The limit of detection is 5.0×10-9 mol·L-1.The binding ratio and binding constant of daunorubicin hydrochloride with BSA were calculated.

Key words: daunorubicin hydrochloride, galactic disk electrode, electrochemical behavior, bovine serum albumin(BSA)

中图分类号:

R927
R96

程红芬, 张勇, 刘杰, 刘艳梅, . 银盘电极盐酸柔红霉素电化学行为及其与BSA的相互作用[J]. 电化学（中英文）, 2009, 15(3): 326-330.

CHENG Hong-fen, ZHang Yong, LIU Jie, LIU Yan-mei. The Electrochemical Behavior of Daunorubicin Hydrochloride on Galactic Disk Electrode and its Reaction with BSA[J]. Journal of Electrochemistry, 2009, 15(3): 326-330.

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链接本文: https://electrochem.xmu.edu.cn/CN/10.61558/2993-074X.2004

https://electrochem.xmu.edu.cn/CN/Y2009/V15/I3/326

参考文献

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银盘电极盐酸柔红霉素电化学行为及其与BSA的相互作用

The Electrochemical Behavior of Daunorubicin Hydrochloride on Galactic Disk Electrode and its Reaction with BSA

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