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电化学(中英文) ›› 2000, Vol. 6 ›› Issue (2): 175-180. 

• 研究论文 • 上一篇    下一篇

检测肠毒素用酶免疫电极动力学参数的测定

董飒英,刘馨苗,王远志,陈光章,吕鸣祥,田波义   

  1. 七二五研究所青岛分部!山东青岛266071,七二五研究所青岛分部!山东青岛266071,七二五研究所青岛分部!山东青岛266071,七二五研究所青岛分部!山东青岛266071,天津大学化工学院!天津300072,天津大学化工学院,天津300072
  • 收稿日期:2000-05-28 修回日期:2000-05-28 出版日期:2000-05-28 发布日期:2000-05-28

Determination of Kinetic Parameters of Enzyme-immunoelectrode for SEC Assay

DONG Sa_Ying 1, LIU Xin_miao 1, WANG Yuan_zhi 1, CHEN Guang_zhang 1, LV Ming_xiang 2, TIAN Bo_yi 2   

  1. ( 1. 725 Research Institute Qingdao Branch, Qingdao 266071, China; 2. College of Chemical Engineering of Tianjin University, Tianjin 300072
  • Received:2000-05-28 Revised:2000-05-28 Published:2000-05-28 Online:2000-05-28

摘要: 应用恒电位极化法探讨了酶免疫电极的响应机理 ,通过旋转圆盘电极确定了生物电极的速度控制步骤 ,根据交流阻抗谱的分析 ,计算了各种生物电极在不同电极电位下的动力学参数 ,并进行了比较分析 .

关键词: 酶免疫电极, 肠毒素, 动力学参数

Abstract: Reaction mechanism of enzyme immunoelectrode is studied by potentiostatic. Rotating disk electrode is used to determine the controlling step of bioelectrode. Under different potentials and different modifying conditions, kinetic parameters of bioelectrodes were measured on the base of electrochemical impendance spectra. At the same time, these parameters are compared and discussed.

Key words: Enzyme immunoelectrode, Staphylococcal Enterotoxin, kinetic parameters

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