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电化学(中英文) ›› 2007, Vol. 13 ›› Issue (2): 140-144.  doi: 10.61558/2993-074X.1796

• 研究论文 • 上一篇    下一篇

纳米二氧化钛对辣根过氧化酶催化作用

朱亚琦;武海;刘辉;马洁;   

  1. 首都师范大学化学系,首都师范大学化学系,首都师范大学化学系,首都师范大学化学系 北京100037,北京100037,北京100037,北京100037
  • 收稿日期:2007-05-28 修回日期:2007-05-28 出版日期:2007-05-28 发布日期:2007-05-28

Catalysis of Nano TiO_2 for Horseradish Peroxidase

ZHU Ya-qi,WU Hai,Liu HIU,MA Jie   

  1. (Department of Chemistry,Capital Normal University,Beijing 100037,China
  • Received:2007-05-28 Revised:2007-05-28 Published:2007-05-28 Online:2007-05-28

摘要: 将辣根过氧化物酶(HRP)固定在二氧化钛(TiO2)纳米颗粒或纳米管修饰玻碳电极(GC)上,形成纳米TiO2微粒/HRP修饰GC电极和TiO2纳米管/HRP修饰GC电极.比较了HRP在纳米TiO2微粒、TiO2纳米管电极上的直接电子转移反应.实验表明,HRP在TiO2纳米管电极表面更能有效地促进它的电活性中心发生电子交换反应.此外,还测定了HRP标记抗体的电化学性能,为抗原抗体免疫反应信号的选择提供了参考依据.

关键词: 二氧化钛纳米管, 电极修饰, 直接电催化, 辣根过氧化酶

Abstract: Horseradish peroxidase immobilized on the surface of titanium dioxide nanoparticles and nanotube modified glassy carbon electrode.The HRP direct electron transfer reaction was compared between TiO2 nanoparticles and nanotube GC electrode.The results demonstrated that the HRP on TiO2 nanotube /GC electrode could catalyze electron exchange reaction between electroactivity center of HRP and electrode.And the research of antibody labeled HRP provides a selected signal for antibody-antigen immunoassay.

Key words: titanium dioxide nanotube, chemically modified electrode, direct electrochemistry, horseradish peroxidase

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