Pt/Au双金属纳米粒子和壳聚糖/二氧化硅溶胶-凝胶杂化膜漆酶传感电极的邻苯二酚检测

doi:10.13208/j.electrochem.130809

电化学（中英文） ›› 2014, Vol. 20 ›› Issue (2): 177-183. doi: 10.13208/j.electrochem.130809

Pt/Au双金属纳米粒子和壳聚糖/二氧化硅溶胶-凝胶杂化膜漆酶传感电极的邻苯二酚检测

娄童芳，屈建莹^*，邢欢欢，杜学萍，董莹

河南大学化学化工学院，环境与分析科学研究所，河南开封 475004

收稿日期:2013-08-09 修回日期:2013-10-25 发布日期:2014-04-17 出版日期:2014-04-28
通讯作者: 屈建莹 E-mail:lilyqujy@yahoo.com.cn; QJY405407@163.com
基金资助:
国家自然科学基金项目（No. 31000065）资助

Preparation of a New Laccase Biosensor based on Bimetallic Pt/Au Particles and Chitosan/silica sol-gel Hybrid Films for Determination of Catechol

LOU Tong-fang, QU Jian-ying^*, XING Huan-huan, DU Xue-ping, DONG Ying

Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China

Received:2013-08-09 Revised:2013-10-25 Online:2014-04-17 Published:2014-04-28
Contact: QU Jian-ying E-mail:lilyqujy@yahoo.com.cn; QJY405407@163.com

摘要/Abstract

摘要： 利用Pt/Au双金属纳米粒子和壳聚糖/二氧化硅（CS-SiO₂）溶胶-凝胶复合材料固定漆酶（Lac），制得漆酶生物电极（Pt-Au-CS-SiO₂-Lac/GC）. 采用循环伏安曲线（CV）研究了邻苯二酚（CC）在该漆酶电极的电化学性能. 实验结果表明，Pt-Au-CS-SiO₂-Lac/GC电极对邻苯二酚的电催化在8×10^-7 ~ 1×10^-4 mol·L^-1浓度范围，其浓度与还原峰电流呈线性关系，相关系数r = 0.9993，检出限7.9×10^-8 mol·L^-1，该检测方法灵敏度高，线性范围宽，稳定性好.

关键词: 漆酶, 壳聚糖/二氧化硅溶胶-凝胶, Pt/Au双金属纳米粒子, 邻苯二酚

Abstract: A novel laccase (Lac) biosensor was fabricated successfully by means of entrapping Lac into bimetallic Pt/Au particles and chitosan/silica (CS-SiO2) sol-gel hybrid films, and then applied to determine catechol (CC) concentration. The experiment results showed that the biosensor can electrocatalyze the reduction of CC, and the peak current increased linearly with the concentration of CC in the range of 8×10^-7 ~ 1×10^-4 mol·L^-1 (r = 0.9993) with a detection limit of 7.9×10^-8 mol·L^-1 by cyclic voltammetric response. The biosensor exhibited good sensitivity, stability and reproducibility, with a broad linear range for potential applications.

Key words: laccase, chitosan/silica sol-gel, bimetallic pt/au particles, catechol

中图分类号:

O657.1

娄童芳, 屈建莹, 邢欢欢, 杜学萍, 董莹. Pt/Au双金属纳米粒子和壳聚糖/二氧化硅溶胶-凝胶杂化膜漆酶传感电极的邻苯二酚检测[J]. 电化学（中英文）, 2014, 20(2): 177-183.

LOU Tong-fang, QU Jian-ying, XING Huan-huan, DU Xue-ping, DONG Ying. Preparation of a New Laccase Biosensor based on Bimetallic Pt/Au Particles and Chitosan/silica sol-gel Hybrid Films for Determination of Catechol[J]. Journal of Electrochemistry, 2014, 20(2): 177-183.

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https://electrochem.xmu.edu.cn/CN/Y2014/V20/I2/177

Pt/Au双金属纳米粒子和壳聚糖/二氧化硅溶胶-凝胶杂化膜漆酶传感电极的邻苯二酚检测

Preparation of a New Laccase Biosensor based on Bimetallic Pt/Au Particles and Chitosan/silica sol-gel Hybrid Films for Determination of Catechol

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