应用交流阻抗法和循环伏安法表征聚苏丹红Ⅲ/GC电极，并研究丹皮酚在该聚合物电极上的电化学行为. 实验表明，聚苏丹红Ⅲ/GC电极对丹皮酚具有良好的电催化作用，在3.0*10-7 ~ 2.2*10-5 mol·L-1浓度范围内，其差示脉冲伏安峰电流随浓度变化呈良好的线性关系，检测限为5.0*10-8 mol·L-1. 该法可用于实际样品中丹皮酚的测定，结果令人满意.
The poly SudanⅢ modified electrode was characterized by electrochemical impedance spectroscopy（EIS）and cyclic voltammetry (CV), while the electrochemical behavior of paeonol was studied by cyclic voltammetry (CV). The result shows that the poly Sudan Ⅲ modified electrode exhibits excellent electrocatalytic effect on the oxidation of paeonol. The peak currents of differential pulse voltammetry are linear to the paeonol concentrations over the range of 3.0*10-7 ~ 2.2*10-5 mol·L-1. The detection limit has been estimated to be 5.0*10-8 mol·L-1. This method can be used for determination of paeonol content in real sample with satisfactory results.
[1] Yang Z S(杨正生), Peng Z H(彭振辉), Yao Q H (姚青海), et al. Progress on pharmacological research of paeonol[J]. Chinese Remedies & Clinics(中国药物与临床), 2011, 11(5): 545-546.
[2] Gao J H(高锦红), Zhang X Y(张学英), Zhu H Y(朱海燕). Determination of paeonol in radix cynanchi paniculati[J]. Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2012, 29(2): 1207-1209.
[3] Li X Y(李向阳), Tu W Q(屠万倩). Determination of paeonol, paeoniflorin and ursolic acid in Liuwei Dihuang Pill by RP-HPLC[J]. Chinese Traditional Patent Medicine(中成药), 2012, 34(2): 277-282.
[4] Dong W, Shen H B, Liu X H, et al. CdSe/ZnS quantum dots based fluorescence quenching method for determination of paeonol[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2011, 78(1): 537-542.
[5] Yu N J(于乃佳), Yu Y(于泳), Hu J B(胡劲波), et al. Studies on voltammetric behavior of paeonol and its application[J]. Journal of Beijing Normal University(Natural Science) (北京师范大学学报自然科学版), 2001, 37(1): 79-82.
[6] Wang Y, Wu J, Li D, et al. Electrochemical oxidation of paeonol and its reaction mechanism[J]. Chinese Journal of Analytical Chemistry, 2006, 34(9): 1331-1334.
[7] Zhang H J, Gao M M, Yang X F. Electrochemical oxidation and detection of paeonol on modified electrode with acetylene black nanoparticles[J]. Colloids and Surfaces B: Biointerfaces, 2011, 87(2): 378-381.
[8] Yang S L, Qu L B, Yang R, et al. Modified glassy carbon electrode with Nafion/MWNTs as a sensitive voltammetric sensor for the determination of paeonol in pharmaceutical and biological samples[J]. Journal of Applied Electrochemistry, 2010, 40(7): 1371-1378.
[9] Chinese pharmacopoeia commission. Pharmacopoeia of People's Republic of China (?)[M]. Beijing: China Medical Science and Technology Press, 2010: 161.
