本文采用一步法制备了1-芘丁酸/石墨烯复合物(PBA/G)，研究了其电化学性质. 采用铁氰化钾和亚铁氰化钾电化学探针测定了电化学阻抗滴定曲线，确定了PBA/G的表观pK_a为6.2. 此外，将葡萄糖氧化酶(GOD)共价键合在PBA/G表面构建了葡萄糖电化学传感器，其电化学响应与葡萄糖浓度（5 mmol L^-1浓度范围内）呈线性，检测限为0.085 mmol L^-1. 实验还测定了固定在PBA/G表面的GOD的表观米氏常数为5.40 mmol L^-1，表明固定化的GOD对葡萄糖有较高的催化活性。

The electrochemical properties of 1-pyrenebutyric acid/graphene composites (PBA/G) obtained by one-step synthesis via π-π stacking was investigated. The electrochemical impedance titration curve shows the surface charge changes as function of solution pH by using ferricyanide/ferrocyanide redox couple as the probe. An apparent pK_a value is estimated as 6.2 according to the impedance titration curve. In addition, a glucose biosensor was constructed by immobilizing glucose oxidase (GOD) on the surface of PBA/G via covalent interaction. This biosensor shows a linear response to glucose within the concentration up to 5 mmol L^-1 with a detection limit of 0.085 mmol L^-1. A small apparent Michaelis-Menten constant (5.40 mmol L^-1) of the immobilized GOD suggests that the immobilized GOD retains its bioactivity and shows high catalytic activity to glucose.