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研究论文

Mb/AuNPs/MWNTs/GC电极对H2O2电催化性能的研究

  • 黄郑隽 ,
  • 彭花萍 ,
  • 查代君 ,
  • 刘爱林 ,
  • 陈伟 ,
  • 林新华 ,
  • 游勇基
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  • 1. 福建医科大学药学院药物分析系,福建 福州 350004; 2. 南昌大学高等研究院,江西 南昌 330031;3. 福建省药品检验所,福建 福州350001

收稿日期: 2012-02-25

  修回日期: 2012-04-10

  网络出版日期: 2012-04-15

基金资助

国家863计划资助项目(No. 2008AA02Z433),国家自然科学基金资助项目(No. 20975021,No. 81171668),福建省高校产学研科技重点项目(No. 2010Y4003),福建省自然科学基金资助项目(No. 2010J06011),福建医科大学校重大项目(No. 09ZD013),福建省教育厅科技项目(No. JA10126,No. JA11110)和福建医科大学博士启动基金(No. 2011BS005)资助

Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction

  • HUANG Zheng-Juan ,
  • PENG Hua-Ping ,
  • CHA Dai-Jun ,
  • LIU Ai-Lin ,
  • CHEN Wei ,
  • LIN Xin-Hua ,
  • YOU Yong-Ji
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  • 1. Department of Pharmaceutical Analysis of the Fujian Medical University,Fuzhou 350004,China;2. Institute for Advanced Study of Nanchang University,Nanchang 330031,China;3. Fujian Provincial Institute for Drug Control,Fuzhou 350001,China

Received date: 2012-02-25

  Revised date: 2012-04-10

  Online published: 2012-04-15

摘要

采用还原法制备了AuNPs/MWNTs复合材料,并构建了氧化还原蛋白质的固定化和生物传感界面AuNPs/MWNTs/GC电极. 以肌红蛋白(Myoglobin,Mb)为例,研究了固定化蛋白质在AuNPs/MWCNTs/GC电极上的直接电化学. 结果表明,AuNPs/MWCNTs复合材料不仅能有效地促进Mb与电极表面的直接电子转移,而且能很好地保持固定化Mb的生物催化活性. Mb/AuNPs/MWCNTs/GC电极对H2O2具有良好的电催化还原性能,其线性响应范围为1 ~ 138 μmol.L-1,检测下限为0.32 μmol.L-1(S/N=3),并具有较低的米氏常数(0.143 mmol.L-1). 该电极操作简单,响应迅速,稳定性和重现性好,有望用于蛋白质的固定化及第三代生物传感器的制备.

本文引用格式

黄郑隽 , 彭花萍 , 查代君 , 刘爱林 , 陈伟 , 林新华 , 游勇基 . Mb/AuNPs/MWNTs/GC电极对H2O2电催化性能的研究[J]. 电化学, 2012 , 18(4) : 377 -382 . DOI: 10.61558/2993-074X.2934

Abstract

A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial (AuNPs/MWNTs), for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin (Mb) as a model, the direct electrochemistry of the immobilized proteins on the AuNPs/MWNTs composite was studied. The results showed that the AuNPs/MWNTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/MWNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H2O2 with a linear range of 1 ~ 138 μmol.L-1,low detection limit of 0.32 μmol.L-1 (S/N=3) and a low apparent Kmapp value of 0.143 mmol.L-1. The simple operation, fast response and well reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.
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