采用还原法制备了AuNPs/MWNTs复合材料，并构建了氧化还原蛋白质的固定化和生物传感界面AuNPs/MWNTs/GC电极. 以肌红蛋白（Myoglobin，Mb）为例，研究了固定化蛋白质在AuNPs/MWCNTs/GC电极上的直接电化学. 结果表明，AuNPs/MWCNTs复合材料不仅能有效地促进Mb与电极表面的直接电子转移，而且能很好地保持固定化Mb的生物催化活性. Mb/AuNPs/MWCNTs/GC电极对H2O2具有良好的电催化还原性能，其线性响应范围为1 ~ 138 μmol.L-1，检测下限为0.32 μmol.L-1（S/N=3），并具有较低的米氏常数（0.143 mmol.L-1）. 该电极操作简单，响应迅速，稳定性和重现性好，有望用于蛋白质的固定化及第三代生物传感器的制备.
黄郑隽
,
彭花萍
,
查代君
,
刘爱林
,
陈伟
,
林新华
,
游勇基
. Mb/AuNPs/MWNTs/GC电极对H2O2电催化性能的研究[J]. 电化学, 2012
, 18(4)
: 377
-382
.
DOI: 10.61558/2993-074X.2934
A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial (AuNPs/MWNTs), for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin (Mb) as a model, the direct electrochemistry of the immobilized proteins on the AuNPs/MWNTs composite was studied. The results showed that the AuNPs/MWNTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/MWNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H2O2 with a linear range of 1 ~ 138 μmol.L-1，low detection limit of 0.32 μmol.L-1 (S/N=3) and a low apparent Kmapp value of 0.143 mmol.L-1. The simple operation, fast response and well reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.
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