以细胞色素c（Cyt c）为模型蛋白，采用表面增强红外吸收光谱监测了三明治结构所吸附的纳米金对氧化还原诱导的Cyt c表面增强红外差谱的改变. 实验表明，在单层Cyt c分子表面组装金纳米粒子，使得血红素的红外差谱特征峰明显增强，这归因于纳米金和血红素之间的电子传递. 金纳米粒子与Cyt c氧化还原活性中心血红素的相互作用加速了蛋白质的电子传递. 这为实现并优化表面吸附蛋白质的直接电化学提供了一种新技术.

The effects of adsorbed gold nanoparticles on the surface-enhanced infrared absorption (SEIRA) difference spectra of redox-induced cytochrome (Cyt) c in AuNPs/Cyt c/Au sandwich architecture were monitored by SEIRA spectroscopy. The results indicated that the intensity of SEIRA difference spectrum for the vibration of the heme was significantly increased due to the adsorption of gold nanoparticles on the Cyt c. This was induced by the electron transfer between the heme and the gold nanoparticles, which also promoted electron transfer of adsorbed protein. This study suggested a new technique for optimizing the electrochemical property of adsorbed protein.