应用循环伏安法研究丹参酮ⅡA在玻碳电极上的电化学行为并建立差示脉冲伏安法测定含量.在pH 4.0醋酸盐缓冲液中,差示脉冲伏安氧化峰电流与丹参酮ⅡA浓度(3.0×10-7~2.0×10-5mol.L-1)呈良好的线性关系,检测限为2.0×10-8mol.L-1.玻碳电极可有效消除样品中其它组分对丹参酮ⅡA测定的干扰,已成功用于实际样品中丹参酮ⅡA含量的直接测定.该方法灵敏度高、检测范围宽,结果令人满意.

The electrochemical behavior of TanshinoneⅡA was investigated by voltammetry at a glassy carbon electrode.And Differential Pulse Voltammetric method for determination of TanshinoneⅡA was developed.In pH 4.0 acetate buffer solution,the relationship between oxidation peak current of Differential Pulse Voltammetric and the concentration of Tanshinone ⅡA was linear in the range of 3.0×10 ~(-7)～2.0×10 ~(–5) mol·L~(-1).The detection limit had been estimated as 2.0×10~(-8) mol·L~(-1).The interference of other components in the preparation could be efficiently eliminated by the glassy carbon electrode.This method can be used for the determination of TanshinoneⅡA in real sample with satisfactory results