应用方波溶出伏安法研究了芦丁在0.1mol.L-1HAc-NaAc缓冲溶液中于玻碳电极上的电化学行为.考察了溶液酸度、沉积电位、沉积时间等因素对芦丁方波溶出伏安行为的影响,优化了实验参数.结果表明,在pH3.0的HAc-NaAc缓冲溶液中,芦丁在-0.46 V产生溶出峰,峰电流与芦丁的浓度在5.0×10-8mol.L-1~1.0×10-6mol.L-1范围内呈现良好的线性关系,相关系数0.997 99,检测限1.1×10-8mol.L-1.可用于药剂中芦丁含量的测定.
The electrochemical behaviors of rutin(Rt) at glassy carbon electrode were studied by square wave stripping voltammeter in the presence of 0.1mol·L-1HAc-NaAc buffer solution(pH=3.0).The sensitive oxidation peak of rutin was observed and which potential peak is 0.46V(vs.SCE).The experimental parameters,such as pH value,deposition potential and deposition time were investigated and selected.The peak currents were proportional to the concentration of rutin in range of 5.0×10-8 mol·L-1~1.0×10-6mol·L-1,and the linear regression coefficient equaled 0.99799,the detection limit was 1.1×10-8 mol·L-1.This method was applied to the determination of rutin content in tablet with satisfactory results.
[1]Alexander V Pastukhov,Lidiya A Levchenko,Anatoli PSadkov.Spectroscopic study on binding of rutin to hu-man serum albumin[J].Journal of Molecular Structure,2007,842:60-66.
[2]Long Xin-hua(龙新华),Yang Mei-hua(杨美华),LiuYao-ming(刘耀明).Content determination of rutin inZhikang Pian by HPLC[J].Chinese Journal of BasicMedicine in Traditional Chinese Medicine(in Chinese),2000,6(9):38-39.
[3]Kang J W,Lu X Q,Zeng H J,et al.Investigation onthe electrochemistry of rutin and its analytical applica-tion[J].Anal Lett,2002,35:677-686.
[4]Tu Yi-feng(屠一锋),Zhou Wen(周文),Ding Yuan-cheng(丁元晨),et al.Use of differential pulse polar-ography for determination of the content of vasodilatationmedicine-rutin[J].Journal of Suzhou University(Na-tional Science),(in Chinese),1992,8(3):361.
[5]Ma Zhi-ru(马志茹),Yuan Zhuo-bin(袁倬斌).Deter-mination of rutin in Japanese pagodatree(sophora japoni-ca)and rutin products by single sweep oscillopolarogra-phy[J].Chinese Traditional and Herbal Drugs(in Chi-nese),1998(2):86-88.
[6]Li Nan-qiang(李南强),Wang Rong-hui(王荣晖).Studies on the voltammetric behaviors of rutin and rareearth ions-rutinsystem[J].Acta Scientiarum NaturaliumUniversitatis Pekinensis,1994,30(4):57.
[7]Yang Yu-fa(杨运发).Voltammetric behavior of rutinon gassy carbon electrode and its determination[J].Chinese Journal of Analytical Chemistry(in Chinese),1996,24(11):1277-1288.
[8]Chen Xin(陈欣),Bao Xiao-yu(包晓玉).Electro-chemical behavior of rutin on glasscarbon electrode[J].Journal of Nanyang Teachers’College(Natural SciencesEdition),(in Chinese),2003,2(3):34-36.
[9]He Jian-Bo,Wang Yan,Deng Ning,et al.Cyclic vol-tammograms obtained from the optical signals:Study ofthe successive electro-oxidations of rutin[J].Electro-chimica Acta,2007,52:6665-6672.
[10]He Jing-Lin,Yang Yu,Yang Xin,et al.β-cyclodex-trin incorporated carbon nanotube-modified electrode asan electrochemical sensor for rutin[J].Sensors andActuators B,2006,114:94-100.
