基于乙酰胆碱酯酶生物传感平台的西维因检测
收稿日期: 2017-12-29
修回日期: 2018-01-30
网络出版日期: 2018-02-26
基金资助
获2017年中国科学院大学生科创计划资助
Acetylcholinesterase Biosensor Platform Based on BP2000 for the Detection of Carbaryl
Received date: 2017-12-29
Revised date: 2018-01-30
Online published: 2018-02-26
张铭函 , 姜俊巧 , 葛君杰 , 邢巍 . 基于乙酰胆碱酯酶生物传感平台的西维因检测[J]. 电化学, 2018 , 24(4) : 303 -308 . DOI: 10.13208/j.electrochem.171229
With the purpose of providing a new method for carbaryl (a pesticide) detection, on the basis of the principle that acetylcholinesterase (AChE) activity can be restrained by carbaryl, an AChE biosensor platform based on BP2000 (as a fixation) was constructed by dropping method. As a result, it revealed that AChE immobilized on BP2000 maintained its catalytic activity for acetylcholine (ATCl), and due to the introduction of the BP2000 material, the effective electrochemical surface area of the modified electrode was enlarged. In addition, the electrochemical oxidation at the modified electrode occurred at low potential (0.630 V) accompanied by proton transmission. The AChE biosensor platform based on BP2000 matrix for carbaryl detection was able to reflect a linear response in the range of 2.0 ng·mL-1 ~ 12.5 ng·mL-1 with the detection limit of 3.15 ng·mL-1. At last, this work will provide a simple method and an efficient matrix in establishing an enzyme electrode of enzymatic biosensor platform and enzymatic fuel cell.
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