利用Pt/Au双金属纳米粒子和壳聚糖/二氧化硅（CS-SiO2）溶胶-凝胶复合材料固定漆酶（Lac），制得漆酶生物电极（Pt-Au-CS-SiO2-Lac/GC）. 采用循环伏安曲线（CV）研究了邻苯二酚（CC）在该漆酶电极的电化学性能. 实验结果表明，Pt-Au-CS-SiO2-Lac/GC电极对邻苯二酚的电催化在8×10-7 ~ 1×10-4 mol·L-1浓度范围，其浓度与还原峰电流呈线性关系，相关系数r = 0.9993，检出限7.9×10-8 mol·L-1，该检测方法灵敏度高，线性范围宽，稳定性好.
A novel laccase (Lac) biosensor was fabricated successfully by means of entrapping Lac into bimetallic Pt/Au particles and chitosan/silica (CS-SiO2) sol-gel hybrid films, and then applied to determine catechol (CC) concentration. The experiment results showed that the biosensor can electrocatalyze the reduction of CC, and the peak current increased linearly with the concentration of CC in the range of 8×10-7 ~ 1×10-4 mol·L-1 (r = 0.9993) with a detection limit of 7.9×10-8 mol·L-1 by cyclic voltammetric response. The biosensor exhibited good sensitivity, stability and reproducibility, with a broad linear range for potential applications.
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