金（111）电极表面浮动磷脂双层膜的原位偏振红外反射光谱研究

doi:10.61558/2993-074X.2610

摘要/Abstract

摘要： 应用电化学原位偏振红外反射光谱法研究了构建于金（111）电极表面的浮动磷脂双层膜。金电极表面先自组装一层巯基葡萄糖单层来增加表面的亲水性，浮动磷脂双层膜通过LB-LS技术构建在巯基葡萄糖单层上.双层膜由双肉豆蔻磷脂酰胆碱（DMPC），胆固醇和神经节苷脂GM1构成.GM1分子中的糖链可以物理吸附在巯基葡萄糖表面，在双层膜和基底间形成一个富含水的隔层.红外光谱表明浮动双层膜中的DMPC分子比传统的支撑双层膜中的DMPC分子有更强的水合作用，证实了双层膜和基底间水层的存在.该浮动双层膜更接近于实际的生物膜体系，并且在金电极表面有宽的电位区间，非常适合于进一步的离子通道蛋白质研究.

关键词: 原位偏振红外反射光谱, 金（111）电极, 浮动磷脂双层膜

Abstract: In situ Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was used to study the structure of a DMPC + cholesterol + GM1 floating bilayer lipid membrane (fBLM) at a Au(111) surface. 1-thio-beta-D-glucose (beta-Tg) was self-assembled onto the Au electrode to increase the overall hydrophilicity of the surface. The fBLM was deposited on the beta-Tg self-assembled monolayer (SAM) using a combination of Langmuir-Blodgett/Langmuir-Schaefer (LB/LS) techniques. The carbohydrate headgroups of the GM1 molecules were physically adsorbed to the beta-Tg SAM forming a water rich cushion between the fBLM and the modified gold substrate. The PM-IRRAS spectra indicate that the DMPC molecules within the fBLM are more hydrated than previous studies involving supported bilayer lipid membranes (sBLM) where the membrane is directly adsorbed onto the surface. The tilt angle of the DMPC acyl chains in the fBLM is smaller than that of the sBLM composed of similar components. The results from this work confirmed that the fBLM is stable over a wide range of electrode potentials and that a water rich region is present between the bilayer and gold electrode surface. The addition of this water region more closely mimics the natural environment of a biological membrane making the fBLM a desirable candidate for future in situ studies involving transmembrane proteins.

Key words: PM-IRRAS, floating bilayer lipid membrane, Au(111) electrode, water rich region

中图分类号:

O6-041

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Zhangfei Su, Annia Kycia, J. Jay Leitch, Jacek Lipkowski. 金（111）电极表面浮动磷脂双层膜的原位偏振红外反射光谱研究[J]. 电化学（中英文）, 2012, 18(5): 387-404.

Zhangfei Su, Annia Kycia, J. Jay Leitch, Jacek Lipkowski. In situ PM-IRRAS Studies of a Floating Bilayer Lipid Membrane at Au(111) Electrode Surface[J]. Journal of Electrochemistry, 2012, 18(5): 387-404.

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https://electrochem.xmu.edu.cn/CN/Y2012/V18/I5/387

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