2,2'-联吡啶In(Ⅲ)配合物与DNA作用的电化学研究

doi:10.61558/2993-074X.1866

电化学（中英文） ›› 2008, Vol. 14 ›› Issue (1): 71-75. doi: 10.61558/2993-074X.1866

2,2'-联吡啶In(Ⅲ)配合物与DNA作用的电化学研究

王俊伟;任建国;周保娟;高筱玲;田燕妮;

山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,山西大学分子科学研究所化学生物学与分子工程教育部重点实验室山西太原030006,山西太原030006,山西太原030006,山西太原030006,山西太原030006

收稿日期:2008-02-28 修回日期:2008-02-28 出版日期:2008-02-28 发布日期:2008-02-28

Study on the Binding mode of In(bpy)Cl_3·H_2O with DNA by the Methods of Electrochemistry

WANG Jun-wei,REN Jian-guo,ZHOU Bao-juan,GAO Xiao-ling,TIAN Yan-ni

(Institute of Molecular Science,Key Laboratory of Chemical Biology andMolecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China

Received:2008-02-28 Revised:2008-02-28 Published:2008-02-28 Online:2008-02-28

摘要/Abstract

摘要： 应用循环伏安法、微分脉冲伏安法和交流阻抗法研究了配合物In(bpy)Cl3.H2O与DNA在Tris-HCl缓冲溶液(pH=7.2)中的相互作用.结果表明:配合物中心In(Ⅲ)离子的循环伏安曲线上呈现一对准可逆的氧化还原波,DNA与配合物作用后,配位中心离子的氧化还原峰电流明显降低,扩散系数减小,电化学反应阻抗增大,式量电位负移,表明该配合物与DNA的作用方式为静电结合.

关键词: In(bpy)Cl3.H2O, DNA, 静电作用

Abstract: The complex In(bpy)Cl3·H2O was synthesized.The binding mode of the complex with DNA was studied by the methods of cyclic voltammetry(CV),differential pulse voltammetry and ac impedance in the buffer Tris-HCl(pH=7.2).A quasi-reversible redox wave for the central Indium ion from the cyclic voltammograms was shown at a PG electrode.It has been found that after the complex acted with DNA,The complex's formal potential negatively shifted from-0.696 V to-0.717 V,the values of the reduction peak current and apparent diffusion coefficient decreased significantly.The results of differential pulse voltammetry were the same as cyclic voltammetry,and the electrochemical reaction resistance values increased from 143 Ω·cm2 to 169 Ω·cm2.The result indicated that the complex In(bpy)Cl3·H2O acted with DNA apparently.The binding mode of the complex with DNA was stable-electricity binding also medium degree exterior bonding mode was manifested.

Key words: In(bpy)Cl3·H2O, calf thymus DNA, stable-electricity binding

中图分类号:

O641.4

王俊伟, 任建国, 周保娟, 高筱玲, 田燕妮, . 2,2'-联吡啶In(Ⅲ)配合物与DNA作用的电化学研究[J]. 电化学（中英文）, 2008, 14(1): 71-75.

WANG Jun-wei, REN Jian-guo, ZHOU Bao-juan, GAO Xiao-ling, TIAN Yan-ni. Study on the Binding mode of In(bpy)Cl_3·H_2O with DNA by the Methods of Electrochemistry[J]. Journal of Electrochemistry, 2008, 14(1): 71-75.

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https://electrochem.xmu.edu.cn/CN/Y2008/V14/I1/71

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2,2'-联吡啶In(Ⅲ)配合物与DNA作用的电化学研究

Study on the Binding mode of In(bpy)Cl_3·H_2O with DNA by the Methods of Electrochemistry

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